US 12,366,578 B2
Fluorescent methods and materials for directed biomarker signal amplification
Brent S. Gaylord, San Diego, CA (US); Janice W. Hong, San Diego, CA (US); Tsu-Ju Fu, Poway, CA (US); Chengjun Sun, Goleta, CA (US); and Russell A. Baldocchi, Encinitas, CA (US)
Assigned to SIRIGEN II LIMITED, Workingham (GB)
Filed by SIRIGEN II LIMITED, Berkshire (GB)
Filed on Apr. 21, 2022, as Appl. No. 17/726,401.
Application 17/726,401 is a continuation of application No. 17/531,027, filed on Nov. 19, 2021.
Application 17/531,027 is a continuation of application No. 16/528,469, filed on Jul. 31, 2019, granted, now 11,209,438, issued on Dec. 28, 2021.
Application 16/528,469 is a continuation of application No. 16/154,541, filed on Oct. 8, 2018, granted, now 10,859,578, issued on Dec. 8, 2020.
Application 16/154,541 is a continuation of application No. 15/799,969, filed on Oct. 31, 2017, granted, now 10,641,777, issued on May 5, 2020.
Application 15/799,969 is a continuation of application No. 15/162,436, filed on May 23, 2016, granted, now 10,126,302, issued on Nov. 13, 2018.
Application 15/162,436 is a continuation of application No. 14/307,258, filed on Jun. 17, 2014, granted, now 9,383,353, issued on Jul. 5, 2016.
Application 14/307,258 is a continuation of application No. 13/195,747, filed on Aug. 1, 2011, granted, now 8,802,450, issued on Aug. 12, 2014.
Application 13/195,747 is a continuation of application No. 11/868,870, filed on Oct. 8, 2007, granted, now 8,158,444, issued on Apr. 17, 2012.
Claims priority of provisional application 60/828,615, filed on Oct. 6, 2006.
Prior Publication US 2022/0260578 A1, Aug. 18, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/58 (2006.01); C08G 61/02 (2006.01); C08G 61/12 (2006.01); G01N 33/542 (2006.01); H10K 85/10 (2023.01)
CPC G01N 33/582 (2013.01) [C08G 61/02 (2013.01); C08G 61/12 (2013.01); G01N 33/542 (2013.01); C08G 2261/3142 (2013.01); C08G 2261/3221 (2013.01); C08G 2261/3241 (2013.01); C08G 2261/3246 (2013.01); C08G 2261/522 (2013.01); C08G 2261/78 (2013.01); C08G 2261/94 (2013.01); H10K 85/115 (2023.02); Y10T 436/13 (2015.01)] 18 Claims
OG exemplary drawing
 
1. A multichromophore-biomolecule conjugate comprising:
a sensor biomolecule covalently linked to a light harvesting multichromophore via a bioconjugation site, wherein the sensor biomolecule is selected from the group consisting of a protein, an antibody and a nucleic acid;
wherein the light harvesting multichromophore is a conjugated polymer having a linear backbone of π-conjugated repeat units of CP1, CP2 and CP3 and comprises the structure:

OG Complex Work Unit Chemistry
wherein:
CP1 is a 2,7-linked fluorene repeat unit;
CP2 comprises an aromatic repeat unit;
CP3 comprises an aromatic repeat unit comprising the sensor biomolecule or a bioconjugation functional group capable of covalently linking to the sensor biomolecule, wherein at least one of the CP3 repeat unit comprises the sensor biomolecule;
CP1, CP2 and CP3 are different from one another and at least one of the CP1, CP2 or CP3 repeat unit is linked by one single bond to at least one other of the CP1, CP2 or CP3 repeat unit;
m is 1 to 10,000;
n is independently 1 to 10,000;
a, b and c are independently 1 to 250; and
wherein the multichromophore does not comprise a biotin group.