US 12,365,890 B2
Optimization of yeast host cells for the production of heterologous proteins
Michelle Oeser, Croydon, NH (US); Janet Fisher, Ossipee, NH (US); and Aaron Argyros, Lebanon, NH (US)
Assigned to DANSTAR FERMENT AG, Zug (CH)
Filed by DANSTAR FERMENT AG, Zug (CH)
Filed on Dec. 19, 2023, as Appl. No. 18/545,514.
Application 18/545,514 is a continuation of application No. 17/277,220, granted, now 11,891,641, previously published as PCT/IB2019/057944, filed on Sep. 19, 2019.
Claims priority of provisional application 62/733,471, filed on Sep. 19, 2018.
Prior Publication US 2024/0182882 A1, Jun. 6, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/81 (2006.01); C12N 9/00 (2006.01); C12N 9/88 (2006.01)
CPC C12N 9/88 (2013.01) [C12N 9/00 (2013.01); C12N 15/81 (2013.01)] 21 Claims
 
1. A recombinant yeast host cell for making an increased amount of a heterologous protein, wherein the recombinant yeast host cell:
(i) (a) has a first heterologous nucleic acid encoding the heterologous protein; and
(b) expresses a variant CYR1 protein; and/or
(ii) is obtained by introducing the first heterologous nucleic acid encoding the heterologous protein in an ancestral yeast host cell that expresses a variant CYR1 protein;
wherein the variant CYR1 protein:
provides to the recombinant yeast host cell or the ancestral yeast host cell a similar intracellular cAMP production in the presence and in the absence of a cAMP stimulus known to stimulate intracellular cAMP production in a control yeast cell, and
comprises the amino acid sequence of SEQ ID NO: 8 with a substitution at position 258, 835, and/or 1435 or a variant thereof having 90% identity with the amino acid sequence of SEQ ID NO: 8 with a substitution at position 258, 835, and/or 1435.