US 12,365,883 B2
Methane monooxygenase enzymes
Elizabeth Jane Clarke, San Francisco, CA (US); Derek Lorin Greenfield, Kinsignton, CA (US); Noah Charles Helman, El Cerrito, CA (US); Stephanie Rhianon Jones, Berkeley, CA (US); and Baolong Zhu, Johnston, IA (US)
Assigned to Industrial Microbes, Inc., Alameda, CA (US)
Filed by Industrial Microbes, Inc., Alameda, CA (US)
Filed on Dec. 21, 2023, as Appl. No. 18/393,317.
Application 18/393,317 is a division of application No. 17/461,102, filed on Aug. 30, 2021, granted, now 11,851,686.
Application 17/461,102 is a continuation of application No. 16/629,018, abandoned, previously published as PCT/US2018/071149, filed on Jul. 6, 2018.
Claims priority of provisional application 62/529,648, filed on Jul. 7, 2017.
Claims priority of provisional application 62/542,838, filed on Aug. 9, 2017.
Claims priority of provisional application 62/566,733, filed on Oct. 2, 2017.
Prior Publication US 2024/0309339 A1, Sep. 19, 2024
Int. Cl. C12N 9/02 (2006.01); C12N 1/21 (2006.01); C12N 15/70 (2006.01); C12N 15/75 (2006.01); C12N 15/77 (2006.01)
CPC C12N 9/0073 (2013.01) [C12N 15/70 (2013.01); C12N 15/75 (2013.01); C12N 15/77 (2013.01); C12Y 114/13025 (2013.01)] 7 Claims
 
1. A mutant soluble diiron monooxygenase system, comprising a mutant hydroxylase gamma subunit comprising an amino acid sequence having at least 80% identity to SEQ ID NO: 5, wherein the mutant hydroxylase gamma subunit comprises a mutation at position 70, wherein the residue position is numbered with respect to SEQ ID NO: 5.