US 12,365,870 B2
Storage stabilizer for extracellular vesicle and storage stabilization method for extracellular vesicle
Masayuki Yamane, Amagasaki (JP); Naoko Imawaka, Amagasaki (JP); Yuji Nakagawa, Osaka (JP); and Kodai Sasamoto, Amagasaki (JP)
Assigned to FUJIFILM Corporation, Tokyo (JP)
Filed by FUJIFILM Wako Pure Chemical Corporation, Osaka (JP)
Filed on Oct. 18, 2021, as Appl. No. 17/504,067.
Application 17/504,067 is a continuation of application No. PCT/JP2020/016738, filed on Apr. 16, 2020.
Claims priority of application No. 2019-080005 (JP), filed on Apr. 19, 2019.
Prior Publication US 2022/0033765 A1, Feb. 3, 2022
Int. Cl. C12N 5/00 (2006.01); C08F 126/10 (2006.01)
CPC C12N 5/00 (2013.01) [C08F 126/10 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A storage stabilization method for an extracellular vesicle, comprising cryopreserving an extracellular vesicle in a presence of a polymer having a monomer unit derived from vinylpyrrolidone,
wherein the polymer is a copolymer containing a monomer unit derived from vinylpyrrolidone and a monomer unit derived from vinyl acetate, or polyvinylpyrrolidone having a weight-average molecular weight of 1,500 to 15,000;
the cryopreserving of an extracellular vesicle is cryopreserving a storage solution comprising the polymer, the extracellular vesicle, and an aqueous solution; and
the concentration of the polymer in the storage solution is 0.001 to 1% (w/v).