US 12,365,869 B2
Systems for production of products to promote nitrogen use efficiency in plants
Luis Francisco Velazquez, Grand Prairie, TX (US); Allana Kay Welsh, Denton, TX (US); Neissa Maryann Pinzon, Frisco, TX (US); Maud Ann Wrightson Hinchee, Little Elm, TX (US); Robert D Chisholm, Mckinney, TX (US); Mohammad Kamrul Hassan, Aubrey, TX (US); Leslie Michelle Perry, Fort Worth, TX (US); Shashi S Rajbanshi, Frisco, TX (US); and Katherine McElhany Williams, Corinth, TX (US)
Assigned to TENFOLD TECHNOLOGIES, LLC, Pilot Point, TX (US)
Filed by Tenfold Technologies, LLC, Pilot Point, TX (US)
Filed on Dec. 15, 2023, as Appl. No. 18/541,917.
Claims priority of provisional application 63/610,535, filed on Dec. 15, 2023.
Claims priority of provisional application 63/510,615, filed on Jun. 27, 2023.
Claims priority of provisional application 63/509,263, filed on Jun. 20, 2023.
Prior Publication US 2024/0425789 A1, Dec. 26, 2024
Int. Cl. C12M 1/00 (2006.01); A01N 63/22 (2020.01); A01N 63/25 (2020.01); A01P 21/00 (2006.01); C05F 11/08 (2006.01); C12M 1/02 (2006.01); C12N 1/20 (2006.01)
CPC C12N 1/20 (2013.01) [A01N 63/22 (2020.01); A01N 63/25 (2020.01); A01P 21/00 (2021.08); C05F 11/08 (2013.01); C12M 23/58 (2013.01); C12M 27/00 (2013.01); C12M 29/18 (2013.01); C12M 47/02 (2013.01)] 43 Claims
OG exemplary drawing
 
1. A bioreactor system comprising:
(a) a conduit comprising a stream of an aqueous feedstock in fluid communication with a first container comprising a volume of a first working fluid and a first container outflow port in fluid communication with the first working fluid and located below the top of the first working fluid, wherein the aqueous feedstock comprises a microbial consortium, wherein the first working fluid comprises an established population of a nitrogen use efficiency-promoting microbial strain;
(b) a second container in fluid communication with the first container, wherein the second container comprises a second working fluid and a second container inflow port in fluid communication with the first container outflow port and arranged to allow hydraulically balanced continuous flow of the first working fluid from the first container outflow port to the second container inflow port driven by gravity, and wherein the second container comprises a second container outflow port located below the top of the second working fluid, and wherein the second container is configured to maintain a population of the nitrogen use efficiency-promoting microbial strain;
(c) one or more additional containers arranged in series with the first container and the second container, wherein each of the one or more additional containers comprises a volume of a working fluid and is in fluid communication with at least one other container in the series, wherein either the second container or at least one of the one or more additional containers comprises a product outflow stream port, wherein at least two of the first container, the second container, or the one or more additional containers are fluidized bed reactors, and wherein the one or more additional containers are configured to maintain a population of the nitrogen use efficiency-promoting microbial strain; and
(d) a product outflow stream in fluid communication with the product outflow stream port, wherein the product outflow stream port is configured to allow hydraulically balanced continuous flow of the product outflow stream driven by gravity.