US 12,364,984 B2
Use of vibrational spectroscopy for microfluidic liquid measurement
Matthias Wagner, Cambridge, MA (US); and John Heanue, Manchester, MA (US)
Assigned to 1087 SYSTEMS, INC., Cambridge, MA (US)
Filed by 1087 Systems, Inc., Cambridge, MA (US)
Filed on Apr. 18, 2024, as Appl. No. 18/639,375.
Application 18/639,375 is a continuation of application No. 17/164,710, filed on Feb. 1, 2021, granted, now 11,965,816.
Application 17/164,710 is a continuation of application No. 16/282,903, filed on Feb. 22, 2019, granted, now 10,908,066, issued on Feb. 2, 2021.
Application 16/282,903 is a continuation of application No. 15/174,681, filed on Jun. 6, 2016, granted, now 10,261,012, issued on Apr. 16, 2019.
Application 15/174,681 is a continuation of application No. 13/894,831, filed on May 15, 2013, granted, now 9,835,552, issued on Dec. 5, 2017.
Application 13/894,831 is a continuation in part of application No. 13/447,647, filed on Apr. 16, 2012, abandoned.
Application 13/894,831 is a continuation in part of application No. 13/298,148, filed on Nov. 16, 2011, granted, now 8,941,062, issued on Jan. 27, 2015.
Application 13/447,647 is a continuation in part of application No. 13/298,148, filed on Nov. 16, 2011, granted, now 8,941,062, issued on Jan. 27, 2015.
Claims priority of provisional application 61/647,041, filed on May 15, 2012.
Claims priority of provisional application 61/628,259, filed on Oct. 27, 2011.
Claims priority of provisional application 61/575,799, filed on Aug. 29, 2011.
Claims priority of provisional application 61/571,051, filed on Jun. 20, 2011.
Claims priority of provisional application 61/519,567, filed on May 25, 2011.
Claims priority of provisional application 61/516,623, filed on Apr. 5, 2011.
Claims priority of provisional application 61/464,775, filed on Mar. 9, 2011.
Claims priority of provisional application 61/456,997, filed on Nov. 16, 2010.
Prior Publication US 2024/0288356 A1, Aug. 29, 2024
Int. Cl. B01L 3/00 (2006.01); B07C 5/34 (2006.01); C12N 5/071 (2010.01); G01N 15/01 (2024.01); G01N 15/10 (2024.01); G01N 15/14 (2024.01); G01N 15/1429 (2024.01); G01N 15/1433 (2024.01); G01N 15/1434 (2024.01); G01N 21/17 (2006.01); G01N 21/35 (2014.01); G01N 21/3563 (2014.01); G01N 29/24 (2006.01); G01N 15/149 (2024.01); G01N 21/65 (2006.01)
CPC B01L 3/502761 (2013.01) [B07C 5/34 (2013.01); C12N 5/0612 (2013.01); G01N 15/14 (2013.01); G01N 15/1427 (2013.01); G01N 15/1429 (2013.01); G01N 15/1433 (2024.01); G01N 15/1434 (2013.01); G01N 15/1436 (2013.01); G01N 15/1459 (2013.01); G01N 15/147 (2013.01); G01N 15/1484 (2013.01); G01N 21/1702 (2013.01); G01N 21/35 (2013.01); G01N 21/3563 (2013.01); G01N 29/2418 (2013.01); B01L 2200/0636 (2013.01); B01L 2200/0652 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0864 (2013.01); G01N 15/01 (2024.01); G01N 2015/1006 (2013.01); G01N 2015/1481 (2013.01); G01N 15/149 (2024.01); G01N 2015/1493 (2013.01); G01N 2015/1497 (2013.01); G01N 2021/653 (2013.01); G01N 2201/0612 (2013.01)] 7 Claims
OG exemplary drawing
 
4. A system for measuring a fluid comprising:
a. a microfluidic chamber (1302) with a measurement volume into which one or more fluids are introduced, the chamber (1302) comprising an illumination region;
b. one or more quantum-cascade laser (QCL) sources (204) configured to emit one or more lights at one or more wavelengths towards at least one of the one or more fluids in the illumination region, wherein after interacting with the at least one of the one or more fluids comprising any respective constituent, the one or more lights are polarized through at least a left circular polarization and a right circular polarization based on a property of the at least one of the one or more fluids inclusive of the any respective constituent;
c. a detector (104) disposed optically in-line with the illumination region, configured to detect the left circular polarization and the right circular polarization; and
d. at least one processor operatively coupled to the detector (104), configured to analyze the left circular polarization and the right circular polarization, measure a vibrational circular dichroism (VCD) differential between the left circular polarization and the right circular polarization, and measure, based on the VCD differential, the property of the at least one of the one or more fluids inclusive of the any respective constituent;
wherein the one or more fluids comprise two or more fluids;
wherein the one or more QCL sources (204) are configured to emit one or more lights towards a first fluid of the two or more fluids and one or more lights towards a second fluid of the two or more fluids;
wherein after interacting with the first fluid comprising any first respective constituent, the one or more lights directed towards the first fluid are polarized through at least a first left circular polarization and a first right circular polarization based on a first property of the first fluid inclusive of the any first respective constituent;
wherein after interacting with the second fluid comprising any second respective constituent, the one or more lights directed towards the second fluid are polarized through at least a second left circular polarization and a second right circular polarization based on a second property of the second fluid inclusive of the any second respective constituent;
wherein the detector (104) is further configured to detect the first left circular polarization and the first right circular polarization of the first fluid and the second left circular polarization and the second right circular polarization of the second fluid;
wherein the at least one processor is further configured to analyze the first left circular polarization and the first right circular polarization of the first fluid and the second left circular polarization and the second right circular polarization of the second fluid, measure a first VCD differential between the first left circular polarization and the first right circular polarization, measure a second VCD differential between the second left circular polarization and the second right circular polarization, and measure, based on a differential between the first VCD differential and the second VCD differential, the first property of the first fluid and the second property of the second fluid.