US 12,037,648 B2
Kits and methods for pedigree division and paternity testing of domestic pigs
Yueyun Ding, Hefei (CN); Xiaoling Ding, Hefei (CN); Zongjun Yin, Hefei (CN); Xudong Wu, Hefei (CN); Xiaodong Zhang, Hefei (CN); Xianrui Zheng, Hefei (CN); Zijing Ling, Hefei (CN); Qiong Chen, Hefei (CN); Yinhui Hou, Hefei (CN); and Chengcheng Kong, Hefei (CN)
Assigned to ANHUI AGRICULTURAL UNIVERSITY, Hefei (CN)
Filed by ANHUI AGRICULTURAL UNIVERSITY, Anhui (CN)
Filed on Jul. 11, 2022, as Appl. No. 17/811,594.
Claims priority of application No. 202110782383.2 (CN), filed on Jul. 12, 2021.
Prior Publication US 2023/0042006 A1, Feb. 9, 2023
Int. Cl. C12Q 1/6888 (2018.01); C12Q 1/6858 (2018.01)
CPC C12Q 1/6888 (2013.01) [C12Q 1/6858 (2013.01); C12Q 2600/124 (2013.01); C12Q 2600/156 (2013.01)] 8 Claims
 
1. A kit for pedigree division and paternity testing of Anqing six-end-white pigs, comprising:
primers configured to amplify 14 simple sequence repeats (SSR) loci which comprises: a S0155 microsatellite locus on chromosome 1, a SW240 microsatellite locus on chromosome 2, a SW72 microsatellite locus on chromosome 3, a S0227 microsatellite locus on chromosome 4, a SW963 microsatellite locus on chromosome 5, a SW122 microsatellite locus on chromosome 6, a S0101 microsatellite locus on chromosome 7, a S0225 microsatellite locus on chromosome 8, a S0386 microsatellite locus on chromosome 11, a S0090 microsatellite locus on chromosome 12, a S0355 microsatellite locus on chromosome 15, a S0026 microsatellite locus on chromosome 16, a SW24 microsatellite locus on chromosome 17, and a SW2156 microsatellite locus on sex chromosome, wherein
the primers comprise: a forward primer sequence of the S0155 microsatellite locus shown in SEQ ID No. 1 and a reverse primer sequence of the S0155 microsatellite locus shown in SEQ ID No. 2; a forward primer sequence of the SW240 microsatellite locus shown in SEQ ID No. 3 and a reverse primer sequence of the SW240 microsatellite locus shown in SEQ ID No. 4; a forward primer sequence of the SW72 microsatellite locus shown in SEQ ID No. 5 and a reverse primer sequence of the SW72 microsatellite locus shown in SEQ ID No. 6; a forward primer sequence of the SW227 microsatellite locus shown in SEQ ID No. 7 and a reverse primer sequence of the SW227 microsatellite locus shown in SEQ ID No. 8; a forward primer sequence of the SW963 microsatellite locus shown in SEQ ID No. 9 and a reverse primer sequence of the SW963 microsatellite locus shown in SEQ ID No. 10; a forward primer sequence of the SW122 microsatellite locus shown in SEQ ID No. 11 and a reverse primer sequence of the SW122 microsatellite locus shown in SEQ ID No. 12; a forward primer sequence of the S0101 microsatellite locus shown in SEQ ID No. 13 and a reverse primer sequence of the S0101 microsatellite locus shown in SEQ ID No. 14; a forward primer sequence of the S0225 microsatellite locus shown in SEQ ID No. 15 and a reverse primer sequence of the S0225 microsatellite locus shown in SEQ ID No. 16; a forward primer sequence of the S0386 microsatellite locus shown in SEQ ID No. 17 and a reverse primer sequence of the S0386 microsatellite locus shown in SEQ ID No. 18; a forward primer sequence of the S0090 microsatellite locus shown in SEQ ID No. 19 and a reverse primer sequence of the S0090 microsatellite locus shown in SEQ ID No. 20; a forward primer sequence of the S0355 microsatellite locus shown in SEQ ID No. 21 and a reverse primer sequence of the S0355 microsatellite locus shown in SEQ ID No. 22; a forward primer sequence of the S0026 microsatellite locus shown in SEQ ID No. 23 and a reverse primer sequence of the S0026 microsatellite locus shown in SEQ ID No. 24; a forward primer sequence of the SW24 microsatellite locus shown in SEQ ID No. 25 and a reverse primer sequence of the SW24 microsatellite locus shown in SEQ ID No. 26; and a forward primer sequence of the SW2156 microsatellite locus shown in SEQ ID No. 27 and a reverse primer sequence of the SW2156 microsatellite locus shown in SEQ ID No. 28.