US 12,037,634 B2
Capsule array devices and methods of use
Benjamin Hindson, Pleasanton, CA (US); Serge Saxonov, Oakland, CA (US); and Michael Schnall-Levin, San Francisco, CA (US)
Assigned to 10X GENOMICS, INC., Pleasanton, CA (US)
Filed by 10X Genomics, Inc., Pleasanton, CA (US)
Filed on Jun. 21, 2021, as Appl. No. 17/353,202.
Application 14/624,468 is a division of application No. 13/966,150, filed on Aug. 13, 2013, abandoned.
Application 17/353,202 is a continuation of application No. 16/519,863, filed on Jul. 23, 2019, granted, now 11,078,522.
Application 16/519,863 is a continuation of application No. 15/975,468, filed on May 9, 2018, abandoned.
Application 15/975,468 is a continuation of application No. 15/598,898, filed on May 18, 2017, abandoned.
Application 15/598,898 is a continuation of application No. 14/624,468, filed on Feb. 17, 2015, granted, now 9,689,024, issued on Jun. 27, 2017.
Claims priority of provisional application 61/683,192, filed on Aug. 14, 2012.
Claims priority of provisional application 61/737,374, filed on Dec. 14, 2012.
Claims priority of provisional application 61/762,435, filed on Feb. 8, 2013.
Claims priority of provisional application 61/800,223, filed on Mar. 15, 2013.
Claims priority of provisional application 61/840,403, filed on Jun. 27, 2013.
Claims priority of provisional application 61/844,804, filed on Jul. 10, 2013.
Prior Publication US 2022/0081704 A1, Mar. 17, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6806 (2018.01); B01J 19/00 (2006.01); B01L 3/00 (2006.01); C12N 15/10 (2006.01)
CPC C12Q 1/6806 (2013.01) [B01J 19/0046 (2013.01); B01L 3/502715 (2013.01); B01L 3/508 (2013.01); B01L 3/523 (2013.01); C12N 15/1065 (2013.01); B01L 2200/0647 (2013.01); B01L 2400/0677 (2013.01); C12Q 2535/122 (2013.01); C12Q 2563/159 (2013.01)] 23 Claims
 
1. A system, comprising:
(i) a bead comprising at least 1,000,000 oligonucleotide barcodes; and
(ii) a microfluidic device comprising:
(a) a first junction of channels configured to form an aqueous mixture comprising the bead comprising the at least 1,000,000 oligonucleotide barcodes and a sample comprising a nucleic acid analyte; and
(b) a second junction of channels configured to contact the aqueous mixture with an immiscible continuous phase, thereby generating a droplet comprising (I) the bead comprising the at least 1,000,000 oligonucleotide barcodes and (II) the nucleic acid analyte.