US 12,360,114 B2
Methods and compositions for protein sequencing
Brian Reed, Madison, CT (US); Jeremy Lackey, Foster City, CA (US); and Haidong Huang, Madison, CT (US)
Assigned to Quantum-Si Incorporated, Branford, CT (US)
Filed by Quantum-Si Incorporated, Branford, CT (US)
Filed on Dec. 10, 2019, as Appl. No. 16/709,024.
Application 16/709,024 is a continuation of application No. 16/686,028, filed on Nov. 15, 2019.
Claims priority of provisional application 62/907,507, filed on Sep. 27, 2019.
Claims priority of provisional application 62/768,076, filed on Nov. 15, 2018.
Prior Publication US 2020/0209255 A1, Jul. 2, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/58 (2006.01); C07K 14/47 (2006.01); C07K 19/00 (2006.01); C12Q 1/6806 (2018.01); G01N 1/28 (2006.01); G01N 21/64 (2006.01); G01N 33/68 (2006.01); G16B 25/10 (2019.01); G16B 30/00 (2019.01); G16B 40/00 (2019.01); G16B 40/10 (2019.01); G16B 50/00 (2019.01); G16B 50/30 (2019.01)
CPC G01N 33/581 (2013.01) [C07K 14/47 (2013.01); C07K 19/00 (2013.01); C12Q 1/6806 (2013.01); G01N 1/28 (2013.01); G01N 21/6428 (2013.01); G01N 33/58 (2013.01); G01N 33/582 (2013.01); G01N 33/6821 (2013.01); G01N 33/6824 (2013.01); G16B 25/10 (2019.02); G16B 40/00 (2019.02); G16B 40/10 (2019.02); G16B 50/30 (2019.02); G01N 2021/6439 (2013.01); G01N 2458/00 (2013.01); G16B 30/00 (2019.02); G16B 50/00 (2019.02)] 22 Claims
 
1. A method of polypeptide sequencing, the method comprising:
(a) detecting a first series of signal pulses indicative of association of one or more amino acid recognition molecules with a first amino acid at a terminus of a single polypeptide molecule;
(b) removing the first amino acid from the terminus of the single polypeptide molecule, wherein a second amino acid is exposed at the terminus of the single polypeptide molecule; and
(c) identifying the second amino acid based at least in part on a first characteristic pattern in the first series of signal pulses, wherein each signal pulse of the first characteristic pattern is indicative of an individual association event between an amino acid recognition molecule and the first amino acid prior to (b).