US 12,360,102 B2
High-throughput assay for cell migration, chemotaxis, and function
Taraka Sai Pavan Grandhi, Collegeville, PA (US); Aaron Tien-Hsin Cheng, Collegeville, PA (US); Jason Elliot Ekert, Collegeville, PA (US); and Terrence Taelim Roh, Collegeville, PA (US)
Assigned to GlaxoSmithKline Intellectual Property Development Limited, (GB)
Appl. No. 18/833,282
Filed by GlaxoSmithKline Intellectual Property Development Limited, Stevenage (GB)
PCT Filed Jan. 26, 2023, PCT No. PCT/IB2023/050684
§ 371(c)(1), (2) Date Jul. 25, 2024,
PCT Pub. No. WO2023/144747, PCT Pub. Date Aug. 3, 2023.
Claims priority of provisional application 63/438,945, filed on Jan. 13, 2023.
Claims priority of provisional application 63/304,083, filed on Jan. 28, 2022.
Prior Publication US 2025/0116657 A1, Apr. 10, 2025
Int. Cl. G01N 33/50 (2006.01); B01L 3/00 (2006.01)
CPC G01N 33/5029 (2013.01) [B01L 3/5027 (2013.01); G01N 33/505 (2013.01); B01L 2200/0694 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/069 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01)] 25 Claims
OG exemplary drawing
 
1. A method of assaying chemotaxis of a cell population, comprising:
providing an assay plate comprising a first two-channel microfluidics unit comprising a first channel and a second channel, wherein the first channel comprises a first gel; wherein the second channel comprises a second gel, wherein the first channel comprises a well at each end and the second channel comprises a well at one end, wherein the first gel in the first channel extends between wells at each end, wherein the second gel in the second channel extends from the well at one end through a central window in which the first and second channels are in fluid communication over a distance; wherein the central window is distal to the well of the second channel;
adding a population of cells to a well of the assay plate, wherein the well is at one end of the first channel and the well is proximal to the well of the second channel;
adding a fluid medium to the well of the second channel and to the distal well of the first channel;
adding a test substance to the distal well of the first channel;
monitoring location of the cells of the population within the central window as the cells traverse the first channel from the well containing the cells toward the well containing the test substance.