US 12,360,042 B2
DUVF-MSI biophotonic analyzer device and methods for detecting pathogens on plants and measuring stress response
Anthony S. Ragone, Wilmington, DE (US)
Assigned to Biospection LLC, Wilmington, DE (US)
Appl. No. 18/001,727
Filed by Biospection LLC, Wilmington, DE (US)
PCT Filed Jun. 22, 2021, PCT No. PCT/US2021/038447
§ 371(c)(1), (2) Date Dec. 14, 2022,
PCT Pub. No. WO2022/005812, PCT Pub. Date Jan. 6, 2022.
Claims priority of provisional application 63/045,578, filed on Jun. 29, 2020.
Prior Publication US 2023/0221254 A1, Jul. 13, 2023
Int. Cl. G01N 21/64 (2006.01); G01N 21/31 (2006.01); G01N 21/47 (2006.01); G01N 21/63 (2006.01); G01N 21/84 (2006.01); G01N 21/88 (2006.01)
CPC G01N 21/6456 (2013.01) [G01N 21/31 (2013.01); G01N 21/474 (2013.01); G01N 21/6486 (2013.01); G01N 2021/635 (2013.01); G01N 2021/6419 (2013.01); G01N 2021/6471 (2013.01); G01N 2021/8466 (2013.01); G01N 2021/8845 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A device for detecting a pathogen on a plant or plant part, the device comprising a light distributing element, a light detection system, a control unit, and a calculating module, wherein:
a) the light distributing element comprises an integrating cavity comprising a first opening, a second opening, and an inner surface on which is disposed a diffuse reflective coating and on which is mounted a plurality of light sources configured to emit light and illuminate an interior of the integrating cavity, wherein:
i) the integrating cavity is configured to distribute the light emitted from the plurality of lights sources through the second opening to illuminate an object sample; and
ii) the second opening is configured to receive reflected light and emitted light from the object sample, the reflected light or emitted light being directed to the first opening;
b) the light detection system is in optical communication with the first opening and the second opening and configured to receive the light reflected by or emitted by the object sample, the light detection system comprising:
i) a camera adapted to supply a representation of recorded images to the calculating module; and
iii) a mirror element in optical alignment with the first opening and the second opening so that an optical axis of the mirror element extends through the first opening and the second opening of the integrating cavity, and wherein the mirror element is configured to divert the light reflected by or emitted by the object sample to the camera;
c) the control unit is configured to cause each of the plurality of light sources to emit light or not emit light;
d) the calculating module is configured to perform spectral calibration of the representation and record measurements of light intensity of the representation;
wherein the plurality of light sources comprises a first light source having a first spectral wavelength in a range of about 250 nm to about 280 nm and a second light source having a second spectral wavelength selected from a range of about 350 nm to about 1000 nm; and
wherein the object sample is a plant or plant part.