US 12,360,037 B2
IR spectroscope cell culture analysis
Mark Hegarty, Glasgow (GB); Matthew J. Baker, Glasgow (GB); and Holly Butler, Glasgow (GB)
Assigned to DXCOVER LIMITED, Glasgow (GB)
Appl. No. 17/792,921
Filed by DXCOVER LIMITED, Glasgow (GB)
PCT Filed Jan. 15, 2021, PCT No. PCT/EP2021/050849
§ 371(c)(1), (2) Date Jul. 14, 2022,
PCT Pub. No. WO2021/144443, PCT Pub. Date Jul. 22, 2021.
Claims priority of application No. 2000670 (GB), filed on Jan. 16, 2020.
Prior Publication US 2023/0068250 A1, Mar. 2, 2023
Int. Cl. G01N 21/3563 (2014.01); C12M 1/26 (2006.01); C12M 1/34 (2006.01); G01N 21/3577 (2014.01); G01N 21/35 (2014.01)
CPC G01N 21/3563 (2013.01) [C12M 33/04 (2013.01); C12M 41/46 (2013.01); G01N 21/3577 (2013.01); G01N 2021/3595 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method for detecting a status of cells within an in vitro cell culture, the method comprising:
providing an Attenuated Total Reflection-Fourier Transform Infrared (ATR-FTIR) spectrum from a test sample obtained from a cell medium within the in vitro cell culture; and
ii) comparing the ATR-FTIR spectrum with an ATR-FTIR spectrum of a control sample or samples, or a sample or samples obtained at an earlier time point of the cell medium within the in vitro cell culture to the test sample, in order to detect any difference between the test and control or earlier samples, which can be correlated with the status of cells within the test sample obtained from the cell medium within the in vitro cell culture, wherein the status of cells within the in vitro cell culture relates to the health of the cells, and wherein the ATR-FTIR spectra are collected at wavenumbers in the region of 400-4000 cm−1.