US 12,360,024 B2
Systems and methods for encapsulation and multi-step processing of biological samples
Linas Mazutis, Vilnius (LT); Greta Stonyte, Vilnius (LT); Karolis Leonavicius, Vilnius (LT); and Ausra Zelvyte, Vilnius (LT)
Assigned to Vilnius University, Vilnius (LT); and Droplet Genomics, UAB, Vilnius (LT)
Filed by Vilnius University, Vilnius (LT); and Droplet Genomics, UAB, Vilnius (LT)
Filed on Nov. 20, 2023, as Appl. No. 18/514,235.
Application 18/514,235 is a division of application No. 16/934,045, filed on Jul. 21, 2020, granted, now 11,860,076.
Claims priority of provisional application 62/863,881, filed on Jun. 20, 2019.
Prior Publication US 2024/0085287 A1, Mar. 14, 2024
Int. Cl. G01N 1/40 (2006.01); B01J 13/04 (2006.01); B01J 13/20 (2006.01); C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01)
CPC G01N 1/4077 (2013.01) [B01J 13/046 (2013.01); B01J 13/206 (2013.01); C12Q 1/6806 (2013.01)] 20 Claims
 
1. A method of barcoding a nucleic acid population arising from a single cell, comprising encapsulating the single cell in a microcapsule having a semipermeable hydrogel shell and an aqueous interior, lysing the single cell such that the nucleic acid population from the single cell is released into the microcapsule, washing the microcapsule in an aqueous solution comprising a barcode population, and forming phosphodiester bonds between barcodes of the barcode population and nucleic acids of the nucleic acid population in the aqueous interior.