US 12,360,023 B2
Method of pretreating blood sample
Hideki Niimi, Toyama (JP); Kazushige Sugie, Toyama (JP); Isao Kitajima, Toyama (JP); Tomohiro Ueno, Toyama (JP); and Atsushi Matsui, Toyama (JP)
Assigned to National University Corporation University of Toyama, (JP)
Filed by LABTECHS Inc., Toyama (JP)
Filed on May 14, 2020, as Appl. No. 16/874,008.
Application 16/874,008 is a continuation of application No. PCT/JP2018/023388, filed on Jun. 20, 2018.
Claims priority of application No. 2017-219547 (JP), filed on Nov. 15, 2017.
Prior Publication US 2020/0355588 A1, Nov. 12, 2020
Int. Cl. G01N 1/40 (2006.01); C12Q 1/00 (2006.01)
CPC G01N 1/4044 (2013.01) [C12Q 1/008 (2013.01)] 4 Claims
 
1. A method of pretreating a blood sample for measuring ATP of a pathogenic microorganism in blood, comprising:
preparing a pellet of platelets and the pathogenic microorganism from the blood sample; and
subjecting the pellet of platelets and the pathogenic microorganism to the following steps (A) to (C) in any order:
(A) digesting cell membrane proteins of platelets with a protease;
(B) swelling the platelets in a hypotonic solution; and
(C) disrupting cell membranes of the platelets with a detergent solution,
wherein the pellet of platelets and the pathogenic microorganism is prepared by subjecting a blood sample collected into an anticoagulant-containing blood collection tube to centrifugation or contact with a separator to remove red and white blood cells and centrifuging the resulting supernatant,
wherein the detergent solution used in the step (C) comprises an anionic detergent (D1) having a linear hydrocarbon in a hydrophobic moiety, and
wherein the anionic detergent (D1) comprises saponin and 0.05 wt % sodium dodecyl sulfate (SDS).