	US 12,359,870 B2
	Method for freeze-drying a hydrogel composition and a freeze-dried hydrogel composition
Markus Nuopponen, Helsinki (FI); Marjo Yliperttula, Espoo (FI); Arto Merivaara, Helsinki (FI); Ossi Korhonen, Hamula (FI); Jacopo Zini, Helsinki (FI); Elle Koivunotko, Helsinki (FI); Lisa Chinello, Padua (IT); Giulia Scapin, Castegnero (IT); Anne Meriluoto, Helsinki (FI); and Lauri Paasonen, Järvenpää (FI)
Assigned to UPM-KYMMENE CORPORATION, Helsinki (FI)
Filed by UPM-Kymmene Corporation, Helsinki (FI)
Filed on Apr. 30, 2021, as Appl. No. 17/245,445.
Prior Publication US 2022/0349651 A1, Nov. 3, 2022
Int. Cl. F26B 5/06 (2006.01); C12N 5/00 (2006.01)

CPC F26B 5/06 (2013.01) [C12N 5/0062 (2013.01); C12N 2513/00 (2013.01); C12N 2533/78 (2013.01)]

9 Claims

1. A method for freeze-drying a hydrogel composition, the method comprising:

providing the hydrogel composition, wherein the hydrogel composition comprises cellulose nanofibrils and/or cellulose nanocrystals, at least one saccharide, at least one amino acid, and biologics, the content of the at least one saccharide in the hydrogel composition being 100-1000 mM, the content of the at least one amino acid in the hydrogel composition being 50-700 mM, the at least one amino acid comprising glycine, leucine, isoleucine, tryptophan, or any combination thereof, the biologics comprising eukaryotic cells, a tissue, an organoid, a mini-organ, extracellular vesicles, or any combination thereof; and

freeze-drying the hydrogel composition, thereby obtaining a freeze-dried hydrogel composition.