US 12,359,256 B2
Circulating RNA signatures specific to preeclampsia
Sarah E. Shultzaberger, San Diego, CA (US); Fiona Kaper, San Diego, CA (US); Sarah Kinnings, San Diego, CA (US); Suzanne Rohrback, San Diego, CA (US); and Carlo Randise-Hinchliff, San Diego, CA (US)
Assigned to ILLUMINA, INC., San Diego, CA (US)
Filed by ILLUMINA, INC., San Diego, CA (US)
Filed on Dec. 29, 2022, as Appl. No. 18/091,186.
Application 18/091,186 is a division of application No. 16/953,480, filed on Nov. 20, 2020, granted, now 11,753,685.
Claims priority of provisional application 62/939,324, filed on Nov. 22, 2019.
Prior Publication US 2023/0135486 A1, May 4, 2023
Int. Cl. C12Q 1/68 (2018.01); C12N 15/10 (2006.01); C12P 19/34 (2006.01); C12Q 1/6883 (2018.01)
CPC C12Q 1/6883 (2013.01) [C12N 15/1096 (2013.01); C12Q 2600/118 (2013.01); C12Q 2600/158 (2013.01)] 16 Claims
 
1. A method of detecting and treating preeclampsia in a subject pregnant human female, the method comprising:
detecting in a biosample from the subject pregnant human female a level of circulating RNA (C-RNA) molecules encoding at least a portion of the protein AKAP2,
wherein the biosample comprises blood, plasma, or serum;
wherein the biosample is obtained from the subject pregnant human female at a gestation age of at least 20 weeks;
wherein the detected level is increased by at least 1.95 fold relative to a level in a gestationally age matched control pregnant human female that does not have preeclampsia; and
wherein the detected level indicates the presence of preeclampsia in the subject pregnant human female; and
providing the subject pregnant human female with a therapeutic intervention for the treatment of preeclampsia selected from the group consisting of antihypertensive medications to lower blood pressure, corticosteroid medications, anticonvulsant medications, bed rest, early delivery, and combinations thereof, and/or treating the subject pregnant human female with a low dose of aspirin, wherein a low dose of aspirin comprises about 50 to about 150 mg per day.