	US 12,359,233 B2
	Production method of (R)-reticuline
Akira Nakagawa, Nonoichi (JP); and Hiromichi Minami, Nonoichi (JP)
Assigned to Fermelanta, Inc., Ishikawa (JP)
Appl. No. 17/622,288
Filed by Fermelanta, Inc., Nonoichi (JP)
PCT Filed Jun. 16, 2020, PCT No. PCT/JP2020/023561 § 371(c)(1), (2) Date Dec. 23, 2021, PCT Pub. No. WO2020/262107, PCT Pub. Date Dec. 30, 2020.
Claims priority of application No. 2019-116701 (JP), filed on Jun. 24, 2019.
Prior Publication US 2022/0251615 A1, Aug. 11, 2022
Int. Cl. C12P 17/12 (2006.01); C12N 1/20 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01); C12N 15/52 (2006.01); C12N 15/70 (2006.01)

CPC C12P 17/12 (2013.01) [C12N 1/20 (2013.01); C12N 9/0042 (2013.01); C12N 9/0071 (2013.01); C12N 9/1029 (2013.01); C12N 15/52 (2013.01); C12N 15/70 (2013.01); C12Y 106/02004 (2013.01); C12Y 114/19 (2013.01); C12Y 203/01037 (2013.01); C12N 2800/101 (2013.01)]

10 Claims

1. A production method of (R)-reticuline comprising:

obtaining a recombinant host cell by inserting, into a host cell,

a gene 1 that comprises a nucleotide sequence having at least 70% homology to the nucleotide sequence of SEQ ID NO: 2, and

a gene 2 that comprises a nucleotide sequence having at least 70% homology to the nucleotide sequence of SEQ ID NO: 3 and encodes a protein having an enzymatic activity of oxidoreductase;

expressing, in the recombinant host cell, a protein encoded by the gene 1 and the protein having the enzymatic activity of oxidoreductase; and

producing (R)-reticuline from(S)-reticuline by using the recombinant host cell,

wherein the host cell is a prokaryote.