US 12,359,215 B2
Modification of ubiquitin binding peptidase genes in plants for yield trait improvement
Lolita George Mathew, Cary, NC (US); and Xiaoyu Zhang, Cary, NC (US)
Assigned to Pairwise Plants Services, Inc., Durham, NC (US)
Filed by Pairwise Plants Services, Inc., Durham, NC (US)
Filed on Aug. 29, 2022, as Appl. No. 17/822,822.
Claims priority of provisional application 63/238,394, filed on Aug. 30, 2021.
Prior Publication US 2023/0074699 A1, Mar. 9, 2023
Int. Cl. C12N 15/82 (2006.01); C12N 15/11 (2006.01)
CPC C12N 15/8261 (2013.01) [C12N 15/11 (2013.01); C12N 15/8213 (2013.01); C12N 2310/20 (2017.05)] 20 Claims
 
1. A soybean plant or part thereof comprising at least one non-natural mutation in an endogenous Ubiquitin Binding Peptidase (DA1) gene encoding a ubiquitin binding peptidase (DA1) polypeptide, wherein the endogenous DA1 gene comprises a nucleotide sequence having at least 80% sequence identity to SEQ ID NO:109, wherein the at least one non-natural mutation comprises a mutation in a region of the endogenous DA1 gene having at least 90% sequence identity to a nucleotide sequence of any one of SEQ ID NOs:112-120 or 127-135, and
wherein the soybean plant comprising the at least one non-natural mutation has a phenotype of one or more improved yield traits as compared to a soybean plant devoid of the at least one non-natural mutation.