US 12,359,214 B2
High expression of animal heme protein in plants
Gaston Paladini, Rosario (AR); Martin Salinas, Funes (AR); Amit Dhingra, College Station, TX (US); Henk Hoogenkamp, Molenhoek (NL); Bruce Williamson Benavides, Moscow, ID (US); Maria Laura Malvino, Camarillo, CA (US); and Balaji Vasudevan, Apex, NC (US)
Assigned to Moolec Science Limited, Warwick (GB)
Filed by MOOLEC SCIENCE LIMITED, Warwick (GB)
Filed on Apr. 26, 2024, as Appl. No. 18/647,942.
Application 18/647,942 is a continuation of application No. PCT/IB2023/056287, filed on Jun. 16, 2023.
Claims priority of provisional application 63/367,299, filed on Jun. 29, 2022.
Prior Publication US 2024/0352475 A1, Oct. 24, 2024
Int. Cl. C12N 15/82 (2006.01); A23J 3/04 (2006.01); A23J 3/22 (2006.01); C07K 14/805 (2006.01)
CPC C12N 15/8257 (2013.01) [A23J 3/04 (2013.01); A23J 3/227 (2013.01); C07K 14/805 (2013.01); C12N 15/8234 (2013.01)] 26 Claims
 
1. A transgenic soybean plant comprising a nucleic acid encoding for a hemeprotein, wherein the nucleic acid is operatively linked to:
a beta-phaseolin (phas) seed-specific promoter at the 5′ end of the nucleic acid encoding for the hemeprotein;
an arc5 terminator at the 3′ end of the nucleic acid encoding for the hemeprotein; and
a Rb7Mar 3′ Matrix Attachment Region at the 3′ end of the arc5 terminator; wherein the hemeprotein is expressed in a seed of the transgenic soybean plant and comprises about 5% to about 30% of the total soluble protein of the seed.