US 12,359,211 B2
Pichia pastoris strains for producing predominantly homogeneous glycan structure
Kurt R. Gehlsen, Tucson, AZ (US); and Thomas G. Chappell, San Marcos, CA (US)
Assigned to Research Corporation Technologies, Inc., Tucson, AZ (US)
Filed by Research Corporation Technologies, Inc., Tucson, AZ (US)
Filed on Nov. 28, 2023, as Appl. No. 18/520,960.
Application 18/520,960 is a continuation of application No. 17/528,619, filed on Nov. 17, 2021, granted, now 11,866,715.
Application 17/528,619 is a continuation of application No. 16/801,466, filed on Feb. 26, 2020, granted, now 11,220,692, issued on Jan. 11, 2022.
Application 16/801,466 is a continuation of application No. 16/404,838, filed on May 7, 2019, granted, now 10,612,033, issued on Apr. 7, 2020.
Application 16/404,838 is a continuation of application No. 15/444,870, filed on Feb. 28, 2017, granted, now 10,329,572, issued on Jun. 25, 2019.
Application 15/444,870 is a continuation of application No. 14/437,683, granted, now 9,617,550, issued on Apr. 11, 2017, previously published as PCT/US2013/066335, filed on Oct. 23, 2013.
Claims priority of provisional application 61/717,423, filed on Oct. 23, 2012.
Prior Publication US 2024/0352472 A1, Oct. 24, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/81 (2006.01); C12N 9/10 (2006.01); C12N 9/24 (2006.01)
CPC C12N 15/815 (2013.01) [C12N 9/1051 (2013.01); C12N 9/2488 (2013.01); C12Y 204/01232 (2013.01); C12Y 302/0113 (2013.01)] 19 Claims
 
1. An engineered stable strain of Pichia pastoris, comprising:
a mutant OCH1 allele comprising a nucleotide sequence encoding a mutant OCH1 protein, wherein said mutant OCH1 protein (i) comprises a catalytic domain comprising amino acid residues 45-404 of the wild type OCH1 protein of the amino acid sequence of SEQ ID NO: 2, or (ii) comprises a catalytic domain comprising at least 90% amino acid sequence identity to the amino acid residues 45-404 of the wild type OCH1 protein of the amino acid sequence of SEQ ID NO: 2, and wherein said mutant OCH1 protein has α-1,6-mannosyltransferase activity, and
wherein said mutant OCH1 protein lacks an N-terminal sequence for targeting the mutant OCH1 protein to the Golgi apparatus.