	US 12,359,207 B2
	Synthetic methylotrophy
Stéphanie Heux, Balma (FR); Alessandro De Simone, Bari (IT); and Jean-Charles Portais, Pibrac (FR)
Assigned to INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE, Toulouse (FR); UNIVERSITE TOULOUSE III-PAUL SABATIER, Toulouse (FR); CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR); and INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT, Paris (FR)
Appl. No. 16/614,878
Filed by INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE, Toulouse (FR); UNIVERSITE TOULOUSE III-PAUL SABATIER, Toulouse (FR); CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR); and INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, Paris (FR)
PCT Filed Jun. 22, 2018, PCT No. PCT/EP2018/066762 § 371(c)(1), (2) Date Nov. 19, 2019, PCT Pub. No. WO2018/234546, PCT Pub. Date Dec. 27, 2018.
Claims priority of application No. 17305781 (EP), filed on Jun. 23, 2017.
Prior Publication US 2023/0183710 A1, Jun. 15, 2023
Int. Cl. C12N 15/52 (2006.01); C12N 9/04 (2006.01); C12N 9/10 (2006.01)

CPC C12N 15/52 (2013.01) [C12N 9/0006 (2013.01); C12N 9/1022 (2013.01); C12Y 101/01244 (2013.01); C12Y 202/01001 (2013.01)]

7 Claims

1. A non-naturally occurring microorganism, which has been engineered to acquire methylotrophy, wherein said microorganism comprises a first enzyme and a second enzyme, wherein said first enzyme is a methanol dehydrogenase (Mdh) enzyme, the nucleic acid sequence of which is selected from the group consisting of: SEQ ID NOs: 34-42 and 45,

wherein said second enzyme is a dihydroxyacetone synthase (Das) enzyme, the nucleic acid sequence of which is SEQ ID NO: 47 or 65,

wherein at least one of said first and second enzymes is heterologous to said microorganism, and

wherein the nucleic acid pair of said first enzyme and said second enzyme is selected from the group consisting of SEQ ID NOs: 36 and 47, 37 and 47, 40 and 47, 34 and 65, 35 and 65, 36 and 65, 37 and 65, 38 and 65, 39 and 65, 40 and 65, 41 and 65, 42 and 65, and 45 and 65.