	US 12,359,161 B2
	Microbiological growth media and methods of using the same
Kate Shara, Nashua, NH (US); Julie Schwedock, Arlington, MA (US); and Sommer Vogel, Lowell, MA (US)
Assigned to RAPID MICRO BIOSYSTEMS, INC., Lowell, MA (US)
Filed by RAPID MICRO BIOSYSTEMS, INC., Lowell, MA (US)
Filed on Jun. 13, 2023, as Appl. No. 18/333,957.
Application 18/333,957 is a division of application No. 17/063,108, filed on Oct. 5, 2020, granted, now 11,732,237.
Application 17/063,108 is a continuation of application No. 15/305,789, granted, now 10,793,823, issued on Oct. 6, 2020, previously published as PCT/US2015/027652, filed on Apr. 24, 2015.
Claims priority of provisional application 61/983,675, filed on Apr. 24, 2014.
Prior Publication US 2023/0323283 A1, Oct. 12, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 1/20 (2006.01); C12N 1/14 (2006.01); C12N 1/16 (2006.01); C12N 1/38 (2006.01); C12Q 1/04 (2006.01)

CPC C12N 1/20 (2013.01) [C12N 1/14 (2013.01); C12N 1/16 (2013.01); C12N 1/38 (2013.01); C12Q 1/045 (2013.01)]

19 Claims

1. A method of preparing a composition, comprising: autoclaving a mixture comprising purified water, casein digest, soybean digest, a phosphate buffer, dextrose, animal tissue digest, yeast extract, hemin, and L-cystine; and adding sheep blood to the mixture, wherein the method does not comprises adding sodium to the mixture.