	US 12,357,924 B2
	*Process for producing enriched fraction from Bacopa monnieri* extract for management of neurodegenerative disorders**
Firoz Hirehal Hussain Mirza, Karnakata (IN); and Singam Setty Nanjundaiah, Karnakata (IN)
Assigned to Star Hi Herbs Pvt Ltd., Karnakata (IN)
Filed by STAR HI HERBS PVT LTD, Karnakata (IN)
Filed on Jan. 7, 2022, as Appl. No. 17/570,918.
Application 17/570,918 is a continuation of application No. PCT/IB2021/059307, filed on Oct. 12, 2021.
Claims priority of application No. 202141036735 (IN), filed on Aug. 13, 2021.
Prior Publication US 2023/0047949 A1, Feb. 16, 2023
Int. Cl. A61K 36/28 (2006.01); B01D 11/02 (2006.01)

CPC B01D 11/0284 (2013.01) [B01D 11/0288 (2013.01); B01D 11/0292 (2013.01); A61K 36/28 (2013.01)]

6 Claims

1. A process of preparation of enriched Bacopa monnieri extract, such said process comprising steps of:

a. extracting Bacopa monnieri with ethanol (101) at volume 1:3 at temperature of 70-75° C. for duration of 3 hours, repeated 3 times;

b. concentrating (102) the ethanol Bacopa monnieri extract at temperature of 70-75° C. under vacuum 760 mmhg;

c. water washing (103) the concentrated Bacopa monnieri at volume 1:2 and separating a layer of concentrated Bacopa monnieri (104) from water;

d. extracting the separated layer containing Bacopa monnieri with Ethyl acetate (105) at temperature of 70-75° C.;

e. concentrating (106) the ethyl acetate Bacopa monnieri extract at temperature of 70-75° C. under vacuum 760 mmhg;

f. washing the concentrated extract followed by drying;

g. milling (111) the dried extract followed by sieving (112) and blending the Bacopa monnieri extract (113) followed by packing (114) an enriched Bacopa monnieri extract.