US 12,357,698 B1
FUS proteolysis-targeting chimera, preparation method and applications thereof
Jun Zhou, Jinan (CN); and Songbo Xie, Jinan (CN)
Assigned to SHANDONG NORMAL UNIVERSITY, Jinan (CN)
Filed by SHANDONG NORMAL UNIVERSITY, Jinan (CN)
Filed on Mar. 7, 2025, as Appl. No. 19/073,405.
Claims priority of application No. 202411258878.5 (CN), filed on Sep. 10, 2024.
Int. Cl. C12N 15/115 (2010.01); A61K 47/54 (2017.01); A61K 47/69 (2017.01)
CPC A61K 47/549 (2017.08) [A61K 47/6921 (2017.08); C12N 15/115 (2013.01); C12N 2310/16 (2013.01)] 7 Claims
 
1. A FUS proteolysis-targeting chimera having a general structure: E-F;
E is an E3 ubiquitin ligase ligand, F is an aptamer specifically binding to FUS protein;
wherein the E3 ubiquitin ligase ligand is a CRBN ligand, and the CRBN ligand is a thalidomide-based CRBN ligand with the structural formula:

OG Complex Work Unit Chemistry
wherein the aptamer specifically binding to FUS protein has a nucleotide sequence as shown in SEQ ID NO: 1.