	US 12,357,659 B2
	Production of megakaryocytes or platelets from monocytes
Zeev Estrov, Houston, TX (US); Srdan Verstovsek, Houston, TX (US); Taghi Manshouri, Houston, TX (US); and Ivo Veletic, Houston, TX (US)
Assigned to BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM, Austin, TX (US)
Filed by BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM, Austin, TX (US)
Filed on Sep. 29, 2021, as Appl. No. 17/488,820.
Claims priority of provisional application 63/085,114, filed on Sep. 29, 2020.
Prior Publication US 2022/0096556 A1, Mar. 31, 2022
Int. Cl. A61K 35/19 (2015.01); A61P 7/04 (2006.01); C12N 5/078 (2010.01)

CPC A61K 35/19 (2013.01) [A61P 7/04 (2018.01); C12N 5/0644 (2013.01)]

11 Claims

1. A method of producing megakaryocytes, comprising:

extracting cells from a tissue of a donor, wherein the tissue is selected from the group consisting of blood and bone marrow;

isolating CD14⁺/CD34⁻ monocytes from the extracted cells; and

culturing the isolated CD14³⁰/CD34⁻ monocytes in vitro in the presence of thrombopoietin (TPO) for a sufficient time to permit differentiation of the CD14⁺/CD34⁻ monocytes into megakaryocytes.