US 12,357,205 B2
Systems and methods for the detection and quantification of ammonia and ammonium in fluids
Marylaura Thomas, Scottsdale, AZ (US); Leslie Thomas, Scottsdale, AZ (US); and Erica Forzani, Scottsdale, AZ (US)
Assigned to Mayo Foundation for Medical Education and Research, Rochester, MN (US); and Arizona Board of Regents on behalf of Arizona State University, Scottsdale, AZ (US)
Filed by Mayo Foundation for Medical Education and Research, Rochester, MN (US); and Arizona Board of Regents on behalf of Arizona State University, Scottsdale, AZ (US)
Filed on Jun. 6, 2024, as Appl. No. 18/736,041.
Application 18/736,041 is a continuation of application No. 18/212,358, filed on Jun. 21, 2023, granted, now 12,178,575.
Application 18/212,358 is a continuation of application No. 16/959,551, granted, now 11,793,431, issued on Oct. 24, 2023, previously published as PCT/US2019/013237, filed on Jan. 11, 2019.
Claims priority of provisional application 62/617,053, filed on Jan. 12, 2018.
Prior Publication US 2024/0335146 A1, Oct. 10, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A61B 5/1455 (2006.01); A61B 5/00 (2006.01); A61B 5/145 (2006.01); A61B 5/20 (2006.01); B01D 53/22 (2006.01)
CPC A61B 5/14552 (2013.01) [A61B 5/14546 (2013.01); A61B 5/201 (2013.01); A61B 5/4866 (2013.01); B01D 53/228 (2013.01); B01D 2259/10 (2013.01)] 12 Claims
OG exemplary drawing
 
8. An analyzer comprising:
a cartridge comprising:
a distributor layer configured to receive a sample of a bodily fluid comprising ammonium (NH4+);
an alkaline layer in contact with the distributor layer and configured to convert at least a portion of the ammonium (NH4+) into ammonia (NH3);
a hydrophobic layer in contact with the alkaline layer and configured to dispel the ammonia (NH3); and
an indicator layer configured to change in color in response to a quantity of the ammonia (NH3); and
a sensor comprising:
at least one light emitting diode configured to illuminate the indicator layer;
at least one photodiode configured to measure absorbance changes of the indicator layer; and
a processor configured to at least one of: quantify the amount of the ammonia (NH3) based on the absorbance changes, identify relative changes in the quantified amount of ammonia (NH3) over time.