	US 12,356,910 B2
	Gene-edited basil plants resistant to downy mildew
Rong Di, East Brunswick, NJ (US); Michael A. Lawton, Highland Park, NJ (US); and James E. Simon, Princeton, NJ (US)
Assigned to Rutgers, The State University of New Jersey, New Brunswick, NJ (US)
Appl. No. 17/914,647
Filed by Rutgers, The State University of New Jersey, New Brunswick, NJ (US)
PCT Filed Mar. 29, 2021, PCT No. PCT/US2021/024697 § 371(c)(1), (2) Date Sep. 26, 2022, PCT Pub. No. WO2021/195630, PCT Pub. Date Sep. 30, 2021.
Claims priority of provisional application 63/000,586, filed on Mar. 27, 2020.
Prior Publication US 2023/0210073 A1, Jul. 6, 2023
Int. Cl. C12N 9/22 (2006.01); A01H 1/00 (2006.01); C12N 15/82 (2006.01)

CPC A01H 1/1255 (2021.01) [C12N 9/22 (2013.01); C12N 15/8282 (2013.01)]

17 Claims

1. A method for increasing Peronospora belbahrii resistance in an Ocimum spp. basil plant, plant part, or cell, comprising:

introducing (i) a gRNA comprising SEQ ID NO: 17, and (ii) a Cas9 protein or gene into the Ocimum spp. basil plant, plant part, or cell, thereby generating a gene-edited plant, plant part, or cell;

wherein the Ocimum spp. basil plant, plant part, or cell comprises a HSK gene comprising at least 95% identity to SEQ ID NO: 1, and the HSK gene comprises SEQ ID NO: 17 adjacent to a PAM site; and

the gene-edited plant, plant part, or cell has increased resistance to Peronospora belbahrii in comparison to a wild type plant, plant part, or cell.