US 12,033,726 B2
Spatially co-registered genomic and imaging (SCORGI) data elements for fingerprinting microdomains
Srinivas C. Chennubhotla, Pittsburgh, PA (US); Albert H. Gough, Glenshaw, PA (US); Andrew M. Stern, Pittsburgh, PA (US); Michael J. Becich, Wexford, PA (US); and Douglass L Taylor, Pittsburgh, PA (US)
Assigned to University of Pittsburgh-Of the Commonwealth System of Higher Education, Pittsburgh, PA (US)
Appl. No. 17/265,954
Filed by UNIVERSITY OF PITTSBURGH-OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION, Pittsburgh, PA (US)
PCT Filed Aug. 21, 2019, PCT No. PCT/US2019/047412
§ 371(c)(1), (2) Date Feb. 4, 2021,
PCT Pub. No. WO2020/046661, PCT Pub. Date Mar. 5, 2020.
Claims priority of provisional application 62/721,018, filed on Aug. 22, 2018.
Prior Publication US 2021/0383894 A1, Dec. 9, 2021
Int. Cl. G16B 40/10 (2019.01); B01L 3/00 (2006.01); G01N 1/28 (2006.01); G01N 1/30 (2006.01); G06F 3/0481 (2022.01); G06F 3/04817 (2022.01); G06F 3/0482 (2013.01); G06T 7/00 (2017.01)
CPC G16B 40/10 (2019.02) [B01L 3/5085 (2013.01); G01N 1/2806 (2013.01); G01N 1/30 (2013.01); G06F 3/04817 (2013.01); G06F 3/0482 (2013.01); G06T 7/0012 (2013.01); G06T 2200/24 (2013.01); G06T 2207/30024 (2013.01); G06T 2207/30072 (2013.01); G06T 2207/30096 (2013.01)] 24 Claims
OG exemplary drawing
 
1. A method of creating a plurality of tissue sections for imaging and genomic analysis, comprising:
obtaining a core from a tissue sample;
obtaining from the core a plurality of imaging data sections and a plurality of genomic data sections in an alternating fashion along a length of the core such that each of the imaging data sections is associated with a respective one of the genomic data sections to form a plurality of serial pairs of adjacent tissue sections, wherein for each serial pair of tissue sections, the imaging data section and the genomic data section are obtained from immediately adjacent portions of the core along the length of the core; and
fixing each of the imaging data sections from the core to a first tissue microarray (TMA) slide and fixing each of the genomic data sections from the core to a second tissue microarray (TMA) slide, wherein for each serial pair of tissue sections the imaging data section of the pair is fixed at a particular location on the first TMA slide and the genomic data section of the pair is fixed at a particular location on the second TMA slide that corresponds to and matches the particular location on the first TMA slide at which the imaging data section of the serial pair is fixed.