US 12,032,149 B2
Imaging method and apparatus for self-luminous object on biological sample film
Yinghao Zhang, Shanghai (CN); and Yan Xi, Shanghai (CN)
Assigned to SHANGHAI E-BLOT PHOTOELECTRIC TECHNOLOGY CO., LTD., Shanghai (CN)
Appl. No. 17/599,529
Filed by SHANGHAI E-BLOT PHOTOELECTRIC TECHNOLOGY CO., LTD., Shanghai (CN)
PCT Filed Mar. 27, 2020, PCT No. PCT/CN2020/081617
§ 371(c)(1), (2) Date Sep. 28, 2021,
PCT Pub. No. WO2020/192755, PCT Pub. Date Oct. 1, 2020.
Claims priority of application No. 201910244792.X (CN), filed on Mar. 28, 2019.
Prior Publication US 2022/0171177 A1, Jun. 2, 2022
Int. Cl. G02B 21/36 (2006.01); G02B 21/10 (2006.01)
CPC G02B 21/365 (2013.01) [G02B 21/10 (2013.01)] 18 Claims
OG exemplary drawing
 
1. An imaging apparatus for self-luminous object on a biological sample film, which is characterized in that the imaging apparatus comprises a housing, a photoelectric conversion element, and an image correction device;
the inside of the housing forms a darkroom space;
the photoelectric conversion element is arranged in the housing;
the photoelectric conversion element is used for obtaining a first dark-field image inside the housing within a preset acquisition duration when the biological sample film is not inserted;
wherein the biological sample film carries a self-luminous object;
the photoelectric conversion element is further used for obtaining a second dark-field image inside the housing within the preset acquisition duration after affixing the biological sample film to the surface of the photoelectric conversion element;
the image correction device is used for performing correction processing on the second dark-field image according to the first dark-field image to obtain a target image corresponding to the self-luminous object;
the imaging apparatus further comprises a light source device, the light source device is arranged in the housing;
the housing comprises a shading cover and a base;
the light source device is arranged on the top position of the inner side of the shading cover;
the photoelectric conversion element is arranged in the base;
the imaging apparatus further comprises a diffuser; the diffuser is fixedly arranged on the inner side of the shading cover and directly under the light source device;
the imaging apparatus further comprises a protective film;
two sides of the protective film are respectively fitted with the biological sample film and the photoelectric conversion element.