US 12,031,992 B2
Methods and systems for controlling oxidative stress
Peter Stroot, Liberty Lake, WA (US)
Assigned to Western Autotroph Company LLC, Liberty Lake, WA (US)
Filed by Western Autotroph Company LLC, Liberty Lake, WA (US)
Filed on Apr. 9, 2021, as Appl. No. 17/227,115.
Application 17/227,115 is a continuation of application No. 16/002,953, filed on Jun. 7, 2018, granted, now 10,976,327.
Application 16/002,953 is a continuation of application No. 14/929,124, filed on Oct. 30, 2015, granted, now 9,995,758, issued on Jun. 12, 2018.
Claims priority of provisional application 62/073,876, filed on Oct. 31, 2014.
Prior Publication US 2021/0333294 A1, Oct. 28, 2021
Int. Cl. G01N 33/84 (2006.01); G01N 21/64 (2006.01); G01N 21/78 (2006.01)
CPC G01N 33/84 (2013.01) [G01N 2021/6439 (2013.01); G01N 21/78 (2013.01); G01N 2800/7009 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method for determining a degree of a manifestation indicative of disease, disorder or indication in a subject, comprising:
adding a first agent to a first volume of a blood sample collected from the subject, the first agent being configured to induce a reactive oxygen species (ROS) response in blood cells of the first volume;
during a first incubation step, incubating: (1) the first volume of blood with the first agent to induce production of ROS in blood cells of the first volume, and (2) a second volume of the blood sample, wherein each of the first and second volumes contains plasma;
during a dilution step, diluting both a first and second volumes, wherein said diluting is configured to reduce a concentration of ROS in the plasma of each of the first and second volumes;
during an isolation step, (1) isolating the plasma of the first volume of blood to produce a first volume of plasma, and (2) isolating the plasma of the second volume of blood to produce a second volume of plasma; and
determining a degree of blood oxidative stress in the subject by comparing the level of ROS response in the first volume and the second volume.