US 12,031,985 B2
Detection of targets
Jayson L. Bowers, Cambridge, MA (US); Michael Cappillino, Haverhill, MA (US); Sadanand Gite, Arlington, MA (US); and Don Straus, Charlestown, MA (US)
Assigned to FIRST LIGHT DIAGNOSTICS, INC., Chelmsford, MA (US)
Filed by First Light Biosciences, Inc., Chelmsford, MA (US)
Filed on Apr. 19, 2019, as Appl. No. 16/389,852.
Claims priority of provisional application 62/660,075, filed on Apr. 19, 2018.
Claims priority of provisional application 62/711,784, filed on Jul. 30, 2018.
Prior Publication US 2019/0324034 A1, Oct. 24, 2019
Int. Cl. G01N 33/569 (2006.01); B01L 3/00 (2006.01); G01N 21/64 (2006.01); G01N 33/53 (2006.01); G01N 33/543 (2006.01); G01N 33/58 (2006.01)
CPC G01N 33/56911 (2013.01) [B01L 3/5025 (2013.01); G01N 21/6428 (2013.01); G01N 33/5302 (2013.01); G01N 33/54326 (2013.01); G01N 33/582 (2013.01); B01L 2300/0681 (2013.01); B01L 2400/043 (2013.01); G01N 2021/6439 (2013.01); G01N 2469/10 (2013.01); G01N 2800/26 (2013.01)] 25 Claims
OG exemplary drawing
 
1. A method for detecting targets comprising:
introducing a biological sample directly to a cassette for analysis;
labeling targets in the sample with a photonic label in a first liquid layer in the cassette; and
separating photonically-labeled targets out of the sample into a second liquid layer within the cassette; and detecting and counting, without magnification, the photonically-labeled targets in the second layer,
wherein the separating step comprises: introducing magnetic particles to the sample to bind to targets; and applying a magnetic field to separate magnetic particle-bound targets from the sample,
wherein the targets are single molecules,
wherein all reagents required for forming the first and second liquid layers and labeling the targets are contained within the cassette to which the biological sample is introduced,
wherein the cassette comprises a channel comprising a neutralization control, wherein the neutralization control comprises detecting and counting targets in a neutralization control sample where neutralization binders are introduced that sequester targets of interest, thereby preventing photonic labeling of the targets.