US 12,031,940 B2
Reagents for potentiometric magnesium ion selective electrode sensors and methods of production and use thereof
Wei Zhang, Needham, MA (US); and Kevin Horan, Raynham, MA (US)
Assigned to Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US)
Filed by Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US)
Filed on Apr. 9, 2021, as Appl. No. 17/226,421.
Application 17/226,421 is a continuation of application No. 16/059,095, filed on Aug. 9, 2018, granted, now 10,983,086.
Application 16/059,095 is a continuation of application No. 15/317,688, granted, now 11,340,190, previously published as PCT/US2015/034668, filed on Jun. 8, 2015.
Claims priority of provisional application 62/011,069, filed on Jun. 12, 2014.
Prior Publication US 2021/0231604 A1, Jul. 29, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 27/416 (2006.01); G01N 27/333 (2006.01); G01N 33/49 (2006.01)
CPC G01N 27/4163 (2013.01) [G01N 27/333 (2013.01); G01N 33/49 (2013.01); G01N 2496/00 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of calibrating a solid-state planar magnesium sensing membrane for a potentiometric ion selective electrode that detects ionized magnesium in a biological sample, wherein the potentiometric ion selective electrode is installed within a clinical diagnostic instrument, the method comprising:
calibrating the magnesium sensing membrane with at least three separate calibration reagents, wherein each of the calibration reagents comprises calcium ions and magnesium ions present in a calcium:magnesium molar ratio in a range of from about 1.5:1 to about 3.25:1; and
wherein the magnesium sensing membrane is subjected to multiple calibrations with the at least three separate calibration reagents and has a stable recovery response for at least four weeks.