US 12,031,911 B2
Sample observation device and sample observation method
Norikazu Sugiyama, Hamamatsu (JP); Masanori Matsubara, Hamamatsu (JP); and Satoshi Yamamoto, Hamamatsu (JP)
Assigned to HAMAMATSU PHOTONICS K.K., Hamamatsu (JP)
Filed by HAMAMATSU PHOTONICS K.K., Hamamatsu (JP)
Filed on Mar. 21, 2022, as Appl. No. 17/699,834.
Application 17/699,834 is a continuation of application No. 16/629,220, granted, now 11,353,402, previously published as PCT/JP2018/015745, filed on Apr. 16, 2018.
Claims priority of application No. 2017-135426 (JP), filed on Jul. 11, 2017.
Prior Publication US 2022/0205913 A1, Jun. 30, 2022
Int. Cl. G01N 21/64 (2006.01)
CPC G01N 21/6428 (2013.01) [G01N 21/6456 (2013.01); G01N 2021/6439 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A device comprising:
a sample container comprising a microplate having a plurality of wells configured to hold a sample into which a solution including a fluorescent substance has been injected, a bottom of each of the wells being formed of a transparent member;
an irradiation unit including a light source and configured to irradiate the well with excitation light, which is planar light, for exciting the fluorescent substance;
an image sensor configured to capture an image of fluorescent light generated in the solution due to the excitation light, and output a plurality of pieces of image data;
an analyzer configured to specify an area in which there is the sample based on the plurality of pieces of image data; and
a scanner configured to drive the sample container in a Y-axis direction, which is a planar direction of the transparent member, at a constant speed;
an image processor configured to generate a fluorescent light image based on the plurality of pieces of image data for the Y-axis direction, and configured to compress the plurality of pieces of image data in a Z-axis direction so as to generate a fluorescent light image,
wherein the excitation light irradiates the solution via the transparent member, and the fluorescent light generated in the solution is captured via the transparent member,
wherein an observation axis for the image sensor is inclined at a predetermined inclination angle with respect to an irradiation surface of the excitation light,
wherein the planar direction of the transparent member and the planar light intersect, and
wherein the image sensor forms the fluorescent image by synthesizing a plurality of compressed image data in the Y-axis direction.