US 12,031,890 B2
Lysis devices having a piezo element and methods
Jeffrey R. Jasperse, Newton, MA (US); Peter Paulicka, Forchheim (DE); and Paul Benson, Quincy, MA (US)
Assigned to Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US)
Appl. No. 18/007,058
Filed by Siemens Healthcare Diagnostics Inc., Tarrytown, NY (US)
PCT Filed Jul. 23, 2021, PCT No. PCT/US2021/042953
§ 371(c)(1), (2) Date Jan. 27, 2023,
PCT Pub. No. WO2022/031459, PCT Pub. Date Feb. 10, 2022.
Claims priority of provisional application 63/060,301, filed on Aug. 3, 2020.
Prior Publication US 2023/0213414 A1, Jul. 6, 2023
Int. Cl. G01N 1/28 (2006.01); B01L 3/00 (2006.01); G01N 29/036 (2006.01); G01N 29/34 (2006.01)
CPC G01N 1/286 (2013.01) [B01L 3/502715 (2013.01); G01N 29/036 (2013.01); G01N 29/348 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/12 (2013.01); B01L 2400/0436 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A lysis device, comprising:
a sample vessel having a microchannel formed therein, the sample vessel having at least one port extending through a surface to the microchannel;
at least one piezo element attached to the surface of the sample vessel; and
a controller having logic to cause the controller to:
emit a first signal including a series of frequencies interleaved with observation periods to the at least one piezo element to cause the at least one piezo element to generate ultrasonic acoustic standing waves in the sample vessel;
receive a second signal indicative of measured vibration signals resulting from the first signal causing the sample vessel to vibrate, the measured vibration signals detected by the at least one piezo element during respective observation periods, each of the measured vibration signals resulting from the first signal having a particular frequency of the series of frequencies and having a decay envelope having a signal strength;
determine the particular frequency of the series of frequencies that results in a particular measured vibration signal of the measured vibration signals having a first decay envelope having a first signal strength greater than a second signal strength of a second decay envelope of at least one other measured vibration signal of the measured vibration signals;
determine a resonant frequency of the sample vessel based on the particular frequency of the series of frequencies that results in the particular measured vibration signal of the measured vibration signals having the first decay envelope having the first signal strength greater than the second signal strength of the second decay envelope of the at least one other measured vibration signal of the measured vibration signals; and
drive the at least one piezo element with one or more third signal at the resonant frequency to lyse cells within a fluid sample.