US 12,031,180 B2
Methods, devices, and systems for analyte detection and analysis
Nathan Beckett, Oakland, CA (US); Gilad Almogy, Palo Alto, CA (US); Nathan Caswell, Sunnyvale, CA (US); Jacob A. Wolf, Oakland, CA (US); Kristopher Barbee, Pleasanton, CA (US); Denis Pristinski, Dublin, CA (US); Mark Pratt, Bozeman, MT (US); Gene Polovy, Redwood City, CA (US); Osip Schwartz, Newark, CA (US); Stephanie Kubecka, San Jose, CA (US); Steven Menchen, Fremont, CA (US); Joseph Anthony, Oakland, CA (US); Jose Martin Sosa, San Jose, CA (US); and Phillip You Fai Lee, South San Francisco, CA (US)
Assigned to Ultima Genomics, Inc., Fremont, CA (US)
Filed by Ultima Genomics, Inc., Newark, CA (US)
Filed on Dec. 6, 2021, as Appl. No. 17/543,521.
Application 17/543,521 is a continuation of application No. 17/308,241, filed on May 5, 2021, granted, now 11,268,143.
Application 17/308,241 is a continuation of application No. 16/953,071, filed on Nov. 19, 2020, granted, now 11,118,223.
Application 16/953,071 is a continuation of application No. PCT/US2020/022816, filed on Mar. 13, 2020.
Application PCT/US2020/022816 is a continuation of application No. 16/677,067, filed on Nov. 7, 2019, granted, now 10,830,703, issued on Nov. 10, 2020.
Application PCT/US2020/022816 is a continuation of application No. 16/677,115, filed on Nov. 7, 2019, granted, now 10,852,518, issued on Dec. 1, 2020.
Application PCT/US2020/022816 is a continuation of application No. 16/445,798, filed on Jun. 19, 2019, granted, now 10,900,078, issued on Jan. 26, 2021.
Claims priority of provisional application 62/914,293, filed on Oct. 11, 2019.
Claims priority of provisional application 62/837,684, filed on Apr. 23, 2019.
Claims priority of provisional application 62/818,549, filed on Mar. 14, 2019.
Prior Publication US 2022/0162696 A1, May 26, 2022
Int. Cl. C12Q 1/6874 (2018.01); G01N 35/10 (2006.01)
CPC C12Q 1/6874 (2013.01) [G01N 35/1002 (2013.01)] 13 Claims
 
1. A method comprising:
(a) dispensing a first solution comprising a plurality of labeled nucleotides of a base type from a first dispenser to an open substrate; and
(b) dispensing a second solution comprising a plurality of unlabeled nucleotides of said base type from a second dispenser to said open substrate, wherein said open substrate is moving relative to said first dispenser and said second dispenser during or subsequent to each of said dispensing in (a) and (b) to disperse said first solution and said second solution, respectively, across said open substrate.