US 12,031,178 B2
Cyclooctatetraene containing dyes and compositions
Xiaolin Wu, Cambridge (GB); Xiaohai Liu, Cambridge (GB); Nikolai Nikolaevich Romanov, Cambridge (GB); Antoine Francais, Cambridge (GB); Patrick McCauley, Cambridge (GB); Michael Callingham, Cambridge (GB); and Carole Anastasi, Cambridge (GB)
Assigned to Illumina Cambridge Limited, Cambridge (GB)
Filed by Illumina Cambridge Limited, Cambridge (GB)
Filed on Feb. 14, 2023, as Appl. No. 18/168,856.
Application 18/168,856 is a continuation of application No. 17/103,169, filed on Nov. 24, 2020, granted, now 11,597,969.
Claims priority of provisional application 62/941,583, filed on Nov. 27, 2019.
Prior Publication US 2023/0265502 A1, Aug. 24, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C09B 57/02 (2006.01); C12Q 1/6869 (2018.01)
CPC C12Q 1/6869 (2013.01) [C09B 57/02 (2013.01)] 28 Claims
 
1. A nucleotide labeled with a fluorescent compound, wherein the fluorescent compound comprises a photo-protecting moiety having the structure of formula (I):

OG Complex Work Unit Chemistry
wherein:
each of R1 and R2 is independently selected from the group consisting of H, —NH2, substituted amino, carboxyl, —C(O)OR5, —C(O)NR6R7, unsubstituted C1-6 alkyl and substituted C1-6 alkyl;
X is —C(═O)—NR4—;
Y is selected from the group consisting of —NR3—, —C(═O)—, —C(═O)—O—, —C(═O)—NR4—, —S(O)2—, —NR3—C(═O)—NR4, and —NR3—C(═S)—NR4—;
Z is absent, unsubstituted C2-6 alkenylene, or unsubstituted C2-6 alkynylene;
each of R3, R4, R6 and R7 is independently H, unsubstituted C1-6 alkyl, or substituted C1-6 alkyl;
R5 is unsubstituted or substituted C1-6 alkyl;
m is an integral number between 1 and 10;
provided that the fluorescent compound is not a cyanine dye, and wherein the fluorescent compound is excitable by a light source having a wavelength between about 350 nm to about 500 nm.