US 12,031,176 B2
Cell analysis device, apparatus, and cell analysis method using same
Masataka Shirai, Tokyo (JP); Tomoyuki Sakai, Tokyo (JP); and Kenko Uchida, Tokyo (JP)
Assigned to HITACHI HIGH-TECH CORPORATION, Tokyo (JP)
Filed by HITACHI HIGH-TECH CORPORATION, Tokyo (JP)
Filed on Jul. 28, 2020, as Appl. No. 16/940,517.
Application 16/940,517 is a division of application No. 15/775,408, granted, now 10,752,941, previously published as PCT/JP2015/083719, filed on Dec. 1, 2015.
Prior Publication US 2020/0354781 A1, Nov. 12, 2020
Int. Cl. C12Q 1/68 (2018.01); C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6809 (2018.01); C12Q 1/6837 (2018.01); C40B 40/08 (2006.01); C40B 50/06 (2006.01); G01N 33/543 (2006.01)
CPC C12Q 1/6837 (2013.01) [C12N 15/1003 (2013.01); C12N 15/1093 (2013.01); C12N 15/1096 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6809 (2013.01); C40B 40/08 (2013.01); C40B 50/06 (2013.01); G01N 33/54306 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A cell analysis method in a cell analysis device which includes:
a solution introduction channel; and
a substrate having a plurality cell capture parts and a plurality of nucleic acid capture parts, the cell capture parts being in contact with the solution introduction channel and having a concavity capable of capturing a single cell, and each of the nucleic acid capture parts being provided in communication with a corresponding cell capture part and configured to capture a nucleic acid extracted from the single cell having been captured by the corresponding cell capture part; and
a discharge channel that is provided adjacent to the nucleic acid capture parts of the substrate and having a three-dimensional porous member,
the cell analysis method comprising the steps of:
introducing a solution containing cells to the solution introduction channel;
applying a negative pressure to the discharge channel to suck the solution from the solution introduction channel and adsorb the cells on the cell capture parts of the substrate and capturing the single cell on the corresponding cell capture part, the negative pressure bringing the substrate into contact with the three-dimensional porous member of the discharge channel;
applying a repulsive force in a direction which separates the cells from the substrate, and
applying a first pressure that is greater than the repulsive force, when the single cell is captured by the corresponding cell capture part, and having a force in the direction from the cell capture part to a corresponding nucleic acid capture part.