US 12,031,171 B2
Fatty acid esters against infections in fermentations
Roel Otto, Gorinchem (NL); Aldana Mariel Ramirez, Amsterdam (NL); and Jenny Eelderink, Gorinchem (NL)
Assigned to PURAC BIOCHEM B.V., Gorinchem (NL)
Appl. No. 16/329,910
Filed by PURAC BIOCHEM BV, Gorinchem (NL)
PCT Filed Sep. 5, 2017, PCT No. PCT/EP2017/072249
§ 371(c)(1), (2) Date Mar. 1, 2019,
PCT Pub. No. WO2018/046500, PCT Pub. Date Mar. 15, 2018.
Claims priority of application No. 16187414 (EP), filed on Sep. 6, 2016.
Prior Publication US 2019/0241916 A1, Aug. 8, 2019
Int. Cl. C12P 7/44 (2006.01); C12N 1/16 (2006.01); C12N 1/20 (2006.01); C12P 7/06 (2006.01); C12P 7/16 (2006.01); C12P 7/18 (2006.01); C12P 7/52 (2006.01); C12P 7/56 (2006.01)
CPC C12P 7/44 (2013.01) [C12N 1/16 (2013.01); C12N 1/20 (2013.01); C12P 7/06 (2013.01); C12P 7/16 (2013.01); C12P 7/18 (2013.01); C12P 7/52 (2013.01); C12P 7/56 (2013.01)] 8 Claims
 
1. A fermentation medium comprising:
an inoculant comprising a culture of at least one production strain of E. coli or Salmonella enterica;
a substrate for microbial growth, the substrate comprising one or more carbohydrates fermentable by the E. coli or Salmonella enterica of the inoculant; and
as an exogenously added ingredient, an antimicrobial agent comprising a lactylate in accordance with Formula 1 or salt thereof in an amount from 0.025 to 0.5 weight %, based on the total weight of the fermentation medium,
(R—(O—CH(CH3)—CO)aO)bM  (Formula 1);
wherein in the Formula 1:
the “R” represents a C4-C18 acyl group, the acyl group having an alkyl or alkenyl chain which may be branched or unbranched;
the “M” represents a proton (H+) or a counter-cation chosen from Li, Na, K, Ca, Mg, Zn, Fe(II), Cu, Mn, Ag, ammonium or substituted ammonium having one or more (C1-4)alkyl optionally substituted with one or more hydroxy;
the “a” is an integer of from 1 to 3;
the “b” is 1 or 2 equaling a valency of M; and
wherein the fermentation medium does not include other antimicrobial agents.