US 12,031,167 B2
Generation of acyl amino acids
Kevin A. Jarrell, Lincoln, MA (US); Gabriel O. Reznik, Bedford, MA (US); Prashanth Vishwanath, Arlington, MA (US); and Michelle Pynn, Andover, MA (US)
Assigned to Modular Genetics, Inc., Lincoln, MA (US)
Filed by Modular Genetics, Inc., Woburn, MA (US)
Filed on Sep. 21, 2021, as Appl. No. 17/481,290.
Application 17/481,290 is a continuation of application No. 16/823,173, filed on Mar. 18, 2020, granted, now 11,155,843.
Application 16/823,173 is a continuation of application No. 15/978,634, filed on May 14, 2018, granted, now 10,640,799, issued on May 5, 2020.
Application 15/978,634 is a continuation of application No. 14/776,805, granted, now 9,970,036, issued on May 15, 2018, previously published as PCT/US2014/029150, filed on Mar. 14, 2014.
Claims priority of provisional application 61/788,346, filed on Mar. 15, 2013.
Prior Publication US 2022/0259626 A1, Aug. 18, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/80 (2006.01); A01N 37/36 (2006.01); C07C 235/06 (2006.01); C12N 9/00 (2006.01); C12P 13/02 (2006.01)
CPC C12P 13/02 (2013.01) [A01N 37/36 (2013.01); C07C 235/06 (2013.01); C12N 9/80 (2013.01); C12N 9/93 (2013.01); C12Y 603/02 (2013.01)] 14 Claims
 
1. A microbial cell engineered to (i) express at least one engineered peptide synthetase, which peptide synthetase synthesizes an acyl amino acid, and (ii) lack endogenous a-keto acid dehydrogenase activity, wherein the cell is a bacterial cell.