	US 12,031,164 B2
	Fusion proteins, recombinant bacteria, and exosporium fragments for plant health
Brian M. Thompson, Creve Coeur, MO (US); Jorg Augustin, Chesterfield, MO (US); and Ashley Siegel, St. Louis, MO (US)
Assigned to Spogen Biotech Inc., St. Louis, MO (US)
Appl. No. 16/648,810
Filed by Spogen Biotech Inc., St. Louis, MO (US)
PCT Filed Sep. 20, 2018, PCT No. PCT/US2018/051995 § 371(c)(1), (2) Date Mar. 19, 2020, PCT Pub. No. WO2019/060574, PCT Pub. Date Mar. 28, 2019.
Claims priority of provisional application 62/560,876, filed on Sep. 20, 2017.
Prior Publication US 2020/0216828 A1, Jul. 9, 2020
Int. Cl. C12N 9/78 (2006.01); A01N 63/22 (2020.01); A23K 10/18 (2016.01); A23K 20/189 (2016.01); A61K 9/00 (2006.01); A61K 35/742 (2015.01); A61K 38/00 (2006.01); C07K 14/32 (2006.01); C12N 9/24 (2006.01); C12N 9/42 (2006.01)

CPC C12N 9/78 (2013.01) [A01N 63/22 (2020.01); A23K 10/18 (2016.05); A23K 20/189 (2016.05); A61K 9/0056 (2013.01); A61K 35/742 (2013.01); C07K 14/32 (2013.01); C12N 9/2402 (2013.01); C12N 9/2434 (2013.01); C12Y 302/01151 (2013.01); C12Y 305/99007 (2013.01); A61K 38/00 (2013.01); C07K 2319/035 (2013.01)]

9 Claims

1. A fusion protein comprising:

(A) a targeting sequence that targets the fusion protein to the exosporium of a recombinant Bacillus cereus family member; and

(B) a phospholipase C enzyme wherein the phospholipase C enzyme comprises: an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 373 or SEQ ID NO: 374 and having phospholipase C activity.