US 12,031,152 B2
Nutrient media for the production of slaughter-free meat
Kalle Lukyan Johnson, Orinda, CA (US); Patricia R. Benton, Huntington Beach, CA (US); Cameron Gentry Copeland, Walnut Creek, CA (US); Sukhdeep Singh Dhadwar, El Cerrito, CA (US); Meri Teresa Firpo, Oakland, CA (US); Marie Elizabeth Gibbons, Emeryville, CA (US); Stephen K. Hsu, Albany, CA (US); Robert Sierra, Berkeley, CA (US); and Kevin John Kayser, Chesterfield, MO (US)
Assigned to UPSIDE FOODS, INC., Berkeley, CA (US)
Filed by Upside Foods, Inc., Berkeley, CA (US)
Filed on Jun. 7, 2021, as Appl. No. 17/341,220.
Claims priority of provisional application 63/035,661, filed on Jun. 5, 2020.
Prior Publication US 2022/0073870 A1, Mar. 10, 2022
Int. Cl. C12N 5/071 (2010.01)
CPC C12N 5/0602 (2013.01) [C12N 2500/14 (2013.01); C12N 2500/24 (2013.01); C12N 2500/32 (2013.01); C12N 2500/34 (2013.01); C12N 2500/36 (2013.01); C12N 2500/38 (2013.01); C12N 2501/105 (2013.01); C12N 2501/11 (2013.01); C12N 2501/113 (2013.01); C12N 2501/115 (2013.01); C12N 2501/117 (2013.01); C12N 2501/12 (2013.01); C12N 2501/135 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/165 (2013.01); C12N 2501/33 (2013.01)] 19 Claims
 
1. A method for formulating an edible nutrient medium for the production of slaughter-free meat for human consumption, comprising:
(a) providing a plurality of ingredients including
(i) one or more of cyanocobalamin, an edible anti-foaming agent, and ferric ammonium citrate; and
(ii) one or more of IGF-1, LR3-IGF1, FGF-1, FGF-2, PDGF, CTGF, EGF, TGFB, BMP, HGF, transferrin, insulin, WNT proteins, interleukins, albumin, VEGF, and homologs thereof; and
(b) mixing the plurality of ingredients in water to form the edible nutrient medium; wherein the nutrient medium does not comprise cobalt chloride, ammonium molybdate, ammonium metavanadate, sodium metavanadate, rubidium chloride, and strontium chloride hexahydrate; thereby forming an edible nutrient medium for culturing an animal cell line suitable for the production of slaughter-free meat.