US 12,031,142 B2
Insecticidal proteins and methods for their use
Jennifer Kara Barry, Ames, IA (US); Ryan Michael Gerber, Apex, NC (US); Lu Liu, Palo Alto, CA (US); Amy Lum, Redwood City, CA (US); Eric Schepers, Port Deposit, MD (US); Nasser Yalpani, Kelowna (CA); and Genhai Zhu, San Jose, CA (US)
Assigned to PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Filed by PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US); and CORTEVA AGRISCIENCE LLC, Indianapolis, IN (US)
Filed on Nov. 19, 2020, as Appl. No. 16/952,193.
Application 16/952,193 is a continuation of application No. 16/809,931, filed on Mar. 5, 2020.
Application 16/809,931 is a continuation of application No. 16/148,668, filed on Oct. 1, 2018, granted, now 10,619,167, issued on Apr. 14, 2020.
Application 16/148,668 is a continuation of application No. 15/116,740, granted, now 10,227,608, issued on Mar. 12, 2019, previously published as PCT/US2015/014824, filed on Feb. 6, 2015.
Claims priority of provisional application 62/051,720, filed on Sep. 17, 2014.
Claims priority of provisional application 61/937,295, filed on Feb. 7, 2014.
Prior Publication US 2021/0139930 A1, May 13, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01); A01N 37/18 (2006.01); A01N 37/46 (2006.01); A01N 63/10 (2020.01); A01N 63/50 (2020.01); A01N 65/04 (2009.01); C07K 14/415 (2006.01)
CPC C12N 15/8286 (2013.01) [A01N 37/18 (2013.01); A01N 37/46 (2013.01); A01N 63/10 (2020.01); A01N 63/50 (2020.01); A01N 65/04 (2013.01); C07K 14/415 (2013.01); Y02A 40/146 (2018.01)] 14 Claims
 
1. A transgenic plant comprising a first heterologous polynucleotide encoding a Pteridophyta insecticidal polypeptide (PtIP) active against a Lepidopteran pest and a second heterologous polynucleotide encoding a bacterial insecticidal polypeptide active against the Lepidopteran pest, wherein the first heterologous polynucleotide and the second heterologous polynucleotide are operably linked to one or more promoter sequences, the PtIP and the bacterial insecticidal polypeptide exhibiting different modes of action against the Lepidopteran pest, wherein said Pteridophyta insecticidal polypeptide comprises an amino acid sequence having at least 71% sequence identity to SEQ ID NO: 1.