US 12,031,139 B2
Stable transformation of a population and a method of biocontainment using haploinsufficiency and underdominance principles
Guy Reeves, Hamburg (DE); and Floyd Reed, Kapolei, HI (US)
Assigned to MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V., Munich (DE)
Filed by MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V., Munich (DE)
Filed on Aug. 12, 2019, as Appl. No. 16/538,602.
Application 16/538,602 is a division of application No. 14/654,450, abandoned, previously published as PCT/EP2013/077856, filed on Dec. 20, 2013.
Claims priority of provisional application 61/740,359, filed on Dec. 20, 2012.
Claims priority of application No. 1223097 (GB), filed on Dec. 20, 2012.
Prior Publication US 2020/0093084 A1, Mar. 26, 2020
Int. Cl. C12N 15/113 (2010.01); A01K 67/033 (2006.01); C12N 15/82 (2006.01)
CPC C12N 15/8218 (2013.01) [A01K 67/033 (2013.01); A01K 67/0333 (2013.01); A01K 67/0339 (2013.01); C12N 15/113 (2013.01); A01K 2207/05 (2013.01); A01K 2217/15 (2013.01); A01K 2227/706 (2013.01); A01K 2267/02 (2013.01); C12N 2310/14 (2013.01)] 11 Claims
 
1. Method for reducing the competitive fitness of an animal hemizygous for a transgenic locus compared to the animal homozygous for the transgenic locus, wherein the animal is Drosophila melanogaster comprising the steps of:
(a) reducing the expression of a haploinsufficient gene in the animal, wherein said reduction is conveyed by a transgenic locus in the animal, wherein said hapoinsufficient gene is RpL14, wherein said transgenic locus comprises an underdominant construct, said underdominant construct encoding and expressing (i) a double-stranded ribonucleic acid (dsRNA) molecule that specifically inhibits expression of the haploinsufficient gene; and (ii) a modified haploinsufficient gene that is resistant to dsRNA inhibition; and
(b) rescuing the reduced expression in the animal, wherein said rescue is conveyed by the same transgenic locus in the animal,
wherein transformation for said reduction and said rescue is carried out simultaneously yielding an animal which is less competitively fit if hemizygous for the transgenic locus than if homozygous for the transgenic locus.