US 12,031,129 B2
Methods and compositions for modulating a genome
Jacob Rosenblum Rubens, Cambridge, MA (US); Geoffrey A. von Maltzahn, Somerville, MA (US); Robert James Citorik, Somerville, MA (US); Barrett Ethan Steinberg, Somerville, MA (US); Donghui Li, Somerville, MA (US); and William Edward Salomon, West Roxbury, MA (US)
Assigned to Flagship Pioneering Innovations VI, LLC, Cambridge, MA (US)
Filed by Flagship Pioneering Innovations VI, LLC, Cambridge, MA (US)
Filed on Dec. 6, 2019, as Appl. No. 16/706,448.
Application 16/706,448 is a continuation of application No. PCT/US2019/048607, filed on Aug. 28, 2019.
Claims priority of provisional application 62/864,924, filed on Jun. 21, 2019.
Claims priority of provisional application 62/850,883, filed on May 21, 2019.
Claims priority of provisional application 62/725,778, filed on Aug. 31, 2018.
Claims priority of provisional application 62/723,886, filed on Aug. 28, 2018.
Prior Publication US 2020/0109398 A1, Apr. 9, 2020
Int. Cl. C12N 15/11 (2006.01); C12N 9/22 (2006.01); C12N 15/85 (2006.01); C12N 15/90 (2006.01)
CPC C12N 15/11 (2013.01) [C12N 9/22 (2013.01); C12N 15/85 (2013.01); C12N 15/907 (2013.01); C12N 2310/20 (2017.05); C12N 2800/00 (2013.01)] 16 Claims
 
1. A composition for modifying DNA, the composition comprising a first engineered RNA and a second engineered RNA, wherein:
(a) the first engineered RNA encodes a polypeptide, wherein the polypeptide comprises (i) a reverse transcriptase domain and (ii) a endonuclease domain, wherein the polypeptide comprises an amino acid sequence according to any of SEQ ID NOs: 1016-1021; and
(b) the second engineered RNA comprises a template RNA, wherein the template RNA comprises (i) a sequence according to SEQ ID NO: 1140 that binds the encoded polypeptide and (ii) a heterologous object sequence, wherein (a) and (b) are separate RNAs.