	US 12,031,128 B2
	Rapid design, build, test, and learn technologies for identifying and using non-viral carriers
Anthony D. Duong, Columbus, OH (US); Danielle J. Huk, Hilliard, OH (US); Cherry Gupta, Columbus, OH (US); Kenneth R. Sims, Jr., Delaware, OH (US); Michael S. Koeris, Irvine, CA (US); Zachary R. Shank, London, OH (US); Ashlee J. Colbert, Blacklick, OH (US); Andrea D. McCue, Columbus, OH (US); Emma K. Schmitz, Columbus, OH (US); Caleb T. Hillrich, Columbus, OH (US); Shannon D. Miller, Hilliard, OH (US); and Joanna L. Hoy, Columbus, OH (US)
Assigned to BATTELLE MEMORIAL INSTITUTE, Columbus, OH (US)
Filed by BATTELLE MEMORIAL INSTITUTE, Columbus, OH (US)
Filed on Apr. 7, 2022, as Appl. No. 17/715,784.
Claims priority of provisional application 63/172,069, filed on Apr. 7, 2021.
Prior Publication US 2022/0333097 A1, Oct. 20, 2022
Int. Cl. C12N 15/10 (2006.01); A61K 48/00 (2006.01); C12N 15/88 (2006.01); C12Q 1/6804 (2018.01); C12Q 1/6816 (2018.01); C12Q 1/682 (2018.01); G01N 33/53 (2006.01)

CPC C12N 15/1065 (2013.01) [A61K 48/0041 (2013.01); A61K 48/0091 (2013.01); C12N 15/88 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/682 (2013.01); C12Q 2525/301 (2013.01); C12Q 2525/313 (2013.01); G01N 33/5308 (2013.01)]

13 Claims

1. A composition comprising:

a) a non-viral delivery vehicle comprising one or more nanoparticle forming polymers, and

b) a nucleic acid construct, wherein the one or more nanoparticle forming polymers are RAFT block copolymers comprising

i) a first terminus comprising a first capping unit derived from a first chain transfer agent in a RAFT copolymerization process, said first chain transfer agent comprising a first reactive functional unit;

ii). a first block prepared from one or more monomer units covalently attached to the first reactive functional unit, and having a molecular weight (M_n) in the range of about 1 kDa to about 200 kDa and a degree of polymerization in the range of about 10 to about 2500;

iii). optionally a second block prepared from one or more monomer units covalently attached to the first block, and having a molecular weight (M_n) in the range of about 1 kDa to about 200 kDa and a degree of polymerization in the range of about 20 to about 2000; and

iv) a second terminus comprising a second capping unit derived from the first or a second chain transfer agent.