	US 12,031,120 B2
	Method for culturing unicellular red algae (URA) with milk permeate
Olivier Cagnac, Libourne (FR); Bruno Gehin, Saint Emilion (FR); and Marion Champeaud, Langoiran (FR)
Assigned to FERMENTALG, Libourne (FR)
Filed by FERMENTALG, Libourne (FR)
Filed on May 2, 2022, as Appl. No. 17/734,621.
Application 17/734,621 is a continuation of application No. 15/780,714, granted, now 11,365,387, previously published as PCT/EP2016/079325, filed on Nov. 30, 2016.
Claims priority of application No. 1561839 (FR), filed on Dec. 4, 2015.
Prior Publication US 2022/0372428 A1, Nov. 24, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 1/12 (2006.01); A23K 10/16 (2016.01)

CPC C12N 1/12 (2013.01) [A23K 10/16 (2016.05)]

16 Claims

1. A method for obtaining a flour comprising unicellular red algae (URA) biomass, said URA being selected from the genus Galdieria, the method comprising:

a) cultivating URA in a medium containing at least one lacteal permeate selected from milk permeate, whey permeate, buttermilk and mixtures thereof; the lacteal permeate comprising lactose as carbon source and organic acids selected from lactic acid and citric acid;

b) collecting the URA biomass from the medium of step a); and

c) processing the collected URA biomass into a flour;

wherein the lactose from lacteal permeate is at an initial concentration in the culture medium ranging between about 0.05 g/L and about 200 g/L; and the URA biomass produced has a URA density of between 20 and 200 g/L dry matter.