US 12,031,118 B2
Reactance and capacitance sensing platform for detecting microorganisms
Patrick Shawn Beaty, Dallastown, PA (US); Michael A. Brasch, Gaithersburg, MD (US); Suneil Hosmane, Franklin Lakes, NJ (US); David Polley, Cockeysville, MD (US); Julie L Rosales, Randallstown, MD (US); and Kerry Lynn Smith, York, PA (US)
Assigned to BECTON, DICKINSON AND COMPANY, Franklin Lakes, NJ (US)
Filed by BECTON DICKINSON AND COMPANY, Franklin Lakes, NJ (US)
Filed on Sep. 14, 2020, as Appl. No. 17/019,802.
Application 17/019,802 is a division of application No. 15/518,989, granted, now 10,808,217, previously published as PCT/US2015/055501, filed on Oct. 14, 2015.
Claims priority of provisional application 62/063,602, filed on Oct. 14, 2014.
Prior Publication US 2020/0407677 A1, Dec. 31, 2020
Int. Cl. C12M 1/34 (2006.01); C12Q 1/02 (2006.01); C12Q 1/04 (2006.01); G01N 27/02 (2006.01); G01N 33/487 (2006.01); G01N 33/49 (2006.01)
CPC C12M 41/36 (2013.01) [C12Q 1/02 (2013.01); C12Q 1/04 (2013.01); G01N 27/02 (2013.01); G01N 33/48785 (2013.01); G01N 27/026 (2013.01); G01N 33/49 (2013.01)] 14 Claims
OG exemplary drawing
 
1. An impedance-based method for detecting microbial growth, the method comprising:
providing a vessel with at least two electrodes disposed therein;
introducing a sample to be monitored for microbial growth in the vessel, the vessel having a volume of at least 10 mL, wherein the electrodes are immersed in the sample;
measuring total impedance of the sample to determine a total ionic composition of the sample over time;
from the measurement, determining an imaginary reactance component of the total impedance over time;
determining changes in the imaginary reactance component over time; and
from the determination of the changes in the imaginary reactance component over time, determining whether microorganism growth has occurred.