US 12,031,117 B2
Perfusion bioreactor system and perfusion cell culture method
Ryann Loren Russell, Drexel Hill, PA (US)
Assigned to CORNING INCORPORATED, Corning, NY (US)
Appl. No. 16/765,666
Filed by CORNING INCORPORATED, Corning, NY (US)
PCT Filed Nov. 20, 2018, PCT No. PCT/US2018/062073
§ 371(c)(1), (2) Date May 20, 2020,
PCT Pub. No. WO2019/104071, PCT Pub. Date May 31, 2019.
Claims priority of provisional application 62/589,669, filed on Nov. 22, 2017.
Prior Publication US 2020/0332242 A1, Oct. 22, 2020
Int. Cl. C12M 1/00 (2006.01); C12M 1/06 (2006.01); C12M 1/12 (2006.01); C12M 3/00 (2006.01)
CPC C12M 29/10 (2013.01) [C12M 21/08 (2013.01); C12M 23/28 (2013.01); C12M 27/02 (2013.01); C12M 29/18 (2013.01); C12M 37/00 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A perfusion bioreactor system comprising:
a vessel having a wall at least partially defining an interior compartment for receiving fluid;
at least one port fluidly connected to at least one of a plurality of external sources;
a small pore membrane device comprising a first external recirculation loop that is fluidly connected to a first outlet from the vessel and a first inlet to the vessel; and
a large pore membrane device comprising a second external recirculation loop that is fluidly connected to a second outlet from the vessel and a second inlet to the vessel;
wherein the small pore membrane device comprises a membrane having an average pore size of between 0.01 lam and 1.0 μm, and
wherein the large pore membrane device comprises a membrane having an average pore size of between 1 μm and 100 μm.