US 12,031,115 B2
Devices and methods for three-dimensional growth of cells
Adrian Ranga, Ixelles (BE); and Sergii Grebeniuk, Heverlee (BE)
Assigned to KATHOLIEKE UNIVERSITEIT LEUVEN, Leuven (BE)
Appl. No. 16/965,878
Filed by Katholieke Universiteit Leuven, Leuven (BE)
PCT Filed Jan. 30, 2019, PCT No. PCT/EP2019/052249
§ 371(c)(1), (2) Date Jul. 29, 2020,
PCT Pub. No. WO2019/149752, PCT Pub. Date Aug. 8, 2019.
Claims priority of application No. 1801582 (GB), filed on Jan. 31, 2018.
Prior Publication US 2021/0054322 A1, Feb. 25, 2021
Int. Cl. C12M 1/12 (2006.01); B01L 3/00 (2006.01); C12M 3/06 (2006.01); C12N 5/00 (2006.01)
CPC C12M 23/16 (2013.01) [B01L 3/502715 (2013.01); C12M 25/14 (2013.01); C12N 5/0075 (2013.01); B01L 2300/163 (2013.01); C12N 2513/00 (2013.01); C12N 2533/54 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A device for the culture of cellular aggregates, the device comprising
a three-dimensional network of interconnected vessels of a biocompatible liquid-and gas-permeable photo-polymerized material, wherein:
the three-dimensional network is connected to a plurality of inlets and a plurality of outlets for the delivery of liquids;
lumens of defined vessels selected from the interconnected vessels within the three-dimensional network are blocked to limit the delivery of liquid by one of the plurality of inlets and one of one of the plurality of outlets to a subregion of the network, thereby defining at least one spatially segregated subregion within and in contact with the three-dimensional network; and
the at least one spatially segregated subregion of the network is connected to another inlet of the plurality of inlets and another outlet of the plurality of outlets, allowing a supply of a liquid to the subregion within the three-dimensional network.