	US 12,031,104 B2
	Method for extracting an oil rich in polyunsaturated fatty acids (PUFA)
Louis Bourles, Bordeaux (FR); Benjamin Seguela, Libourne (FR); Emma Caderby, Cenon (FR); and Hywel Griffiths, Libourne (FR)
Assigned to FERMENTALG, Libourne (FR)
Appl. No. 17/275,848
Filed by FERMENTALG, Libourne (FR)
PCT Filed Sep. 13, 2019, PCT No. PCT/EP2019/074461 § 371(c)(1), (2) Date Mar. 12, 2021, PCT Pub. No. WO2020/053375, PCT Pub. Date Mar. 19, 2020.
Claims priority of application No. 1858293 (FR), filed on Sep. 14, 2018.
Prior Publication US 2022/0041953 A1, Feb. 10, 2022
Int. Cl. C11B 1/10 (2006.01); A23D 9/04 (2006.01); A23L 33/12 (2016.01); A61K 8/36 (2006.01); A61K 36/02 (2006.01); C11B 3/00 (2006.01)

CPC C11B 1/10 (2013.01) [A23D 9/04 (2013.01); A23L 33/12 (2016.08); A61K 8/361 (2013.01); A61K 36/02 (2013.01); C11B 3/00 (2013.01); A23V 2002/00 (2013.01); A61K 2800/10 (2013.01)]

18 Claims

1. A process for extracting a PUFA-rich oil from a biomass of an oil producing organism comprising said oil, the process comprising the steps:

(a) of cell lysis of the said oil-producing organism biomass, and

(b) of mechanical separation of the oil from the lysed biomass of step (a) and recovery of the crude oil,

wherein the cell lysis (a) comprises two parts:

(a1) a first part carried out at a first temperature, followed by

(a2) a second part of continued lysis at a second temperature lower than the temperature of the first part, wherein the temperature of step (a2) of the second part of lysis is at least 10° C. lower than the temperature of the first part (a1).